Abstract | BACKGROUND: RESULTS: Cerebrospinal fluid (CSF) and serum samples of dogs during the acute phase of SRMA (SRMA) were tested for IL-6, VEGF and TGF- β1. Results were compared to those of dogs affected with SRMA during treatment (SRMA Th) and during relapse (SRMA R), to dogs with other meningoencephalomyelitides (ME), with miscellaneous non-inflammatory diseases of the CNS (CNS-Mix), with idiopathic epilepsy (IE), with systemic inflammatory diseases (Syst. Infl.) and with healthy dogs (Healthy). Concentrations of IL-6 and VEGF in CSF were significantly elevated in the SRMA group compared to the other disease categories (p<0.05). The CSF concentrations of TGF-β1 were increased in SRMA group, but statistically significant differences were found only in comparison with Healthy and CNS-Mix groups. No differences were detected in the serum concentrations of TGF-β1 between the different groups. In untreated SRMA patients, a positive correlation (rSpear = 0.3549; P=0.0337) between concentrations of TGF-β1 and IgA concentration was found in CSF, while concentrations of IL-6 and VEGF in CSF positively correlated with the degree of pleocytosis (rSpear=0.8323; P<0.0001 and rSpear=0.5711; P=0.0166, respectively). CONCLUSIONS: Our results suggest that these three signalling proteins are biomarkers of disease activity in SRMA. VEGF might play an important role in the development of a systemic arteritis. TGF-β1 is considered to be involved in the excessive IgA production, while IL-6 in the pleocytosis. The combined intrathecal increase of TGF-β1 and IL-6 detected in SRMA could possibly force CD4 progenitors to differentiate towards the newly described Th17 lymphocyte subset and enhance the autoimmune response.
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Authors | Arianna Maiolini, Meike Otten, Marion Hewicker-Trautwein, Regina Carlson, Andrea Tipold |
Journal | BMC veterinary research
(BMC Vet Res)
Vol. 9
Pg. 23
(Feb 04 2013)
ISSN: 1746-6148 [Electronic] England |
PMID | 23379382
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Acute-Phase Proteins
- Biomarkers
- Immunoglobulin A
- Interleukin-6
- Transforming Growth Factor beta1
- Vascular Endothelial Growth Factor A
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Topics |
- Acute-Phase Proteins
(physiology)
- Animals
- Arteritis
(blood, cerebrospinal fluid, physiopathology, veterinary)
- Biomarkers
(blood, cerebrospinal fluid)
- Dog Diseases
(blood, cerebrospinal fluid, physiopathology)
- Dogs
- Immunoglobulin A
(blood)
- Inflammation
(blood, cerebrospinal fluid, physiopathology, veterinary)
- Interleukin-6
(blood, cerebrospinal fluid, physiology)
- Meningitis
(blood, cerebrospinal fluid, physiopathology, veterinary)
- Transforming Growth Factor beta1
(blood, cerebrospinal fluid, physiology)
- Vascular Endothelial Growth Factor A
(blood, cerebrospinal fluid, physiology)
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