Abstract |
Fibrosarcoma is a rare malignant tumor originating from fibroblasts. Transforming growth factor beta 2 (TGFβ2) has been established to regulate processes correlated to fibrosarcoma tumorigenesis. In this study, we investigated the possible participation of syndecan-2 (SDC-2), a cell membrane heparan sulfate (HS) proteoglycan on these TGFβ2 functions. Our results demonstrate that the inhibition of SDC-2 expression by short interfering RNA (siSDC2) abolished TGFβ2-dependent HT1080 cell adhesion (P ≤ 0.01). In parallel, the downregulation of SDC-2 significantly inhibited TGFβ2-induced Smad2 phosphorylation (P ≤ 0.01). The immunoflourescence signal of TGF receptor III as well as its protein expression was decreased in SDC-2-deficient cells. The enhancement of adhesion molecules integrin β1 (P ≤ 0.01) and focal adhesion kinase expression, induced by TGFβ2 treatment (P ≤ 0.001), was markedly inhibited in SDC-2-defficient cells (P ≤ 0.01). Conclusively, the obtained data suggest that SDC-2 modulates TGFβ2 transcriptional regulation via Smad signaling to facilitate fibrosarcoma cell adhesion.
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Authors | Maria Mytilinaiou, Artan Bano, Dragana Nikitovic, Aikaterini Berdiaki, Kallirroi Voudouri, Alexandra Kalogeraki, Nikos K Karamanos, George N Tzanakakis |
Journal | IUBMB life
(IUBMB Life)
Vol. 65
Issue 2
Pg. 134-43
(Feb 2013)
ISSN: 1521-6551 [Electronic] England |
PMID | 23297089
(Publication Type: Journal Article)
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Copyright | Copyright © 2013 International Union of Biochemistry and Molecular Biology, Inc. |
Chemical References |
- Actins
- Fibronectins
- Integrin beta1
- RNA, Small Interfering
- Receptors, Transforming Growth Factor beta
- SDC2 protein, human
- SMAD2 protein, human
- Smad2 Protein
- Transforming Growth Factor beta2
- Syndecan-2
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Topics |
- Actins
(metabolism)
- Cell Adhesion
- Cell Line, Tumor
- Fibronectins
(metabolism)
- Fibrosarcoma
- Gene Expression
- Gene Knockdown Techniques
- Humans
- Integrin beta1
(genetics, metabolism)
- Protein Multimerization
- Protein Transport
- RNA, Small Interfering
(genetics)
- Receptors, Transforming Growth Factor beta
(genetics, metabolism)
- Smad2 Protein
(metabolism)
- Syndecan-2
(genetics, metabolism)
- Transforming Growth Factor beta2
(physiology)
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