Abstract | BACKGROUND AND AIMS: METHODS: Biopsy samples were taken from NERD patients and healthy control subjects without heartburn. The expression level of nociceptors, neuropeptides, and their receptors were assessed by real-time RT-PCR and enzyme immunoassay. Localization of substance P and CGRP in the esophageal mucosa was determined by immunohistochemical staining. RESULTS: Expression of mRNA for TRPV1 and PAR2 was significantly elevated in the esophageal mucosa of NERD patients. Substance P protein level and its receptor NK1R mRNA also increased in NERD patients. A positive correlation between the substance P protein level and reflux symptoms was observed. Immunohistochemical study revealed the presence of substance P-positive nerves in the lamina propria of the esophagus. CONCLUSIONS:
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Authors | Norimasa Yoshida, Masaaki Kuroda, Takahiro Suzuki, Kazuhiro Kamada, Kazuhiko Uchiyama, Osamu Handa, Tomohisa Takagi, Toshikazu Yoshikawa, Hirofumi Kuramoto |
Journal | Digestive diseases and sciences
(Dig Dis Sci)
Vol. 58
Issue 8
Pg. 2237-43
(Aug 2013)
ISSN: 1573-2568 [Electronic] United States |
PMID | 22899239
(Publication Type: Journal Article)
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Chemical References |
- Neuropeptides
- RNA, Messenger
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Topics |
- Aged
- Esophagus
(metabolism)
- Female
- Gastroesophageal Reflux
(pathology)
- Gene Expression Regulation
(physiology)
- Humans
- Male
- Middle Aged
- Mucous Membrane
(metabolism)
- Neuropeptides
(genetics, metabolism)
- Nociceptors
(metabolism)
- Pain
(metabolism)
- RNA, Messenger
(genetics, metabolism)
- Real-Time Polymerase Chain Reaction
- Reverse Transcriptase Polymerase Chain Reaction
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