Are circulating autoantibodies to ABCC3 transporter a potential biomarker for lung cancer?

Abstract

PURPOSE:

The present study was undertaken to test circulating autoantibody to ATP-binding cassette C3 (ABCC3) transporter in order to confirm whether anti-ABCC3 antibody could serve as a biomarker for early diagnosis of lung cancer.

METHODS:

This study recruited 275 patients (178 males and 97 females) with non-small cell lung cancer (either squamous carcinoma or adenocarcinoma) and 226 control subjects (134 males and 92 females) well matched in age and smoking history. Anti-ABCC3 IgA and IgG were determined using an enzyme-linked immunosorbent assay (ELISA) approach that was developed in house with the human leukocyte antigen class II (HLA-II) restricted antigens.

RESULTS:

Mann-Whitney U test showed that the IgG antibody level was significantly higher in female patients with adenocarcinoma than female controls (Z = -4.34, P < 0.001) and that the IgA antibody level was significantly higher in male patients with squamous carcinoma than male controls (Z = -3.12, P = 0.002). Pearson's Chi-square (χ(2)) test showed that female patients with adenocarcinoma had a significantly higher positive rate for IgG autoantibody than female controls (χ ( 2 ) = 8.73, P = 0.003). The ELISA sensitivity against a specificity of >95 % was 18.1 % for IgG assay in female patients and 18.0 % for IgA assay in male patients. The inter-assay deviation was 10.6 % for IgG assay and 14.5 % for IgA assay.

CONCLUSIONS:

Circulating autoantibodies to ABCC3 transporter may be a potential biomarker that can be added to a panel of existing biomarkers for early diagnosis and prognosis of lung cancer although the gender differences should be taken into account.

• Authors: Linlin Liu, Nian Liu, Baogang Liu, Yanming Yang, Qi Zhang, Weijing Zhang, Pengyue Yu, Yonglong Jin, Jia Guo, Songlei Guan, Shilong Sun, Lining Miao, Jun Wei
• Journal: Journal of cancer research and clinical oncology (J Cancer Res Clin Oncol) Vol. 138 Issue 10 Pg. 1737-42 (Oct 2012) ISSN: 1432-1335 [Electronic] Germany
• PMID: 22699933 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Autoantibodies
• Biomarkers, Tumor
• Immunoglobulin A
• Immunoglobulin G
• Multidrug Resistance-Associated Proteins
• multidrug resistance-associated protein 3

Topics

• Adenocarcinoma (blood, diagnosis, immunology, pathology)
• Adenocarcinoma of Lung
• Autoantibodies (blood, immunology)
• Biomarkers, Tumor (blood, immunology)
• Carcinoma, Non-Small-Cell Lung (blood, diagnosis, immunology, pathology)
• Carcinoma, Squamous Cell (blood, diagnosis, immunology, pathology)
• Early Detection of Cancer (methods)
• Female
• Humans
• Immunoglobulin A (blood, immunology)
• Immunoglobulin G (blood, immunology)
• Lung Neoplasms (blood, diagnosis, immunology, pathology)
• Male
• Middle Aged
• Multidrug Resistance-Associated Proteins (immunology)
• Protein Transport