The protective effects of ozone therapy in a rat model of acetaminophen-induced liver injury.

Abstract

OBJECTIVES:

Acetaminophen (APAP) overdose may cause acute liver injury. Ozone therapy (OT) is shown to reduce inflammation and necrosis in several entities. Thus, we have designed this study to evaluate the efficacy of OT in a rat model of APAP-induced liver injury.

METHODS:

Twenty-seven Sprague-Dawley rats were divided into three groups: sham, APAP and APAP+OT groups. In the APAP and the APAP+OT groups, liver injury was induced by oral administration of 1 g/kg APAP. The APAP+OT group received a single dose ozone/oxygen mixture (0.7 mg/kg) intraperitoneally 1h after APAP administration. All animals were killed at 24 hour after APAP administration. Blood samples and liver tissues were harvested to determine liver injury and oxidative stress parameters. Liver tissues and blood samples were obtained for biochemical and histopathological analyses.

RESULTS:

APAP administration caused necrosis in the liver after 24h. The degrees of liver necrosis of the APAP group were higher than the other groups (in both p<0.05, respectively). In the APAP+OT group, liver antioxidant enzymes activities were significantly higher than the APAP group (p<0.05), but were lower than the sham group (p<0.05). In the sham group, serum neopterin, a marker of cell-mediated immunity, concentrations (4.8±1.2 nmol/L) were lower than the APAP (14.7±1.4 nmol/L) and APAP+OT groups (7.5±2.4 nmol/L) (in both p<0.05, respectively).

CONCLUSION:

Our results showed that OT prevented liver necrosis in rats and reduced neopterin levels. These findings suggest that the use of OT as an adjuvant therapy which might improve the outcome in APAP induced liver injury.

• Authors: Husamettin Gul, Bulent Uysal, Erdinc Cakir, Halil Yaman, Enis Macit, Ali Osman Yildirim, Yusuf Emrah Eyi, Umit Kaldirim, Emin Oztas, Emin Ozgur Akgul, Tuncer Cayci, Mehmet Ozler, Turgut Topal, Sukru Oter, Ahmet Korkmaz, Mehmet Toygar, Suzi Demirbag
• Journal: Environmental toxicology and pharmacology (Environ Toxicol Pharmacol) Vol. 34 Issue 1 Pg. 81-6 (Jul 2012) ISSN: 1872-7077 [Electronic] Netherlands
• PMID: 22459800 (Publication Type: Journal Article)
• Copyright: Copyright © 2012 Elsevier B.V. All rights reserved.

Chemical References

• Analgesics, Non-Narcotic
• Nitrates
• Nitrites
• Protective Agents
• Acetaminophen
• Malondialdehyde
• Ozone
• Neopterin
• Glutathione Peroxidase
• Superoxide Dismutase
• Aspartate Aminotransferases
• Alanine Transaminase

Topics

• Acetaminophen (toxicity)
• Alanine Transaminase (metabolism)
• Analgesics, Non-Narcotic (toxicity)
• Animals
• Aspartate Aminotransferases (metabolism)
• Chemical and Drug Induced Liver Injury (drug therapy, metabolism, pathology)
• Disease Models, Animal
• Glutathione Peroxidase (metabolism)
• Liver (drug effects, metabolism, pathology)
• Male
• Malondialdehyde (metabolism)
• Necrosis (chemically induced, drug therapy, metabolism, pathology)
• Neopterin (blood)
• Nitrates (blood)
• Nitrites (blood)
• Ozone (therapeutic use)
• Protective Agents (therapeutic use)
• Rats
• Rats, Sprague-Dawley
• Superoxide Dismutase (metabolism)