Abstract | AIMS: METHODS AND RESULTS: PR expression was examined by immunohistochemistry (n=143) and kinetic reverse transcription-polymerase chain reaction (RT-PCR) from formalin-fixed and paraffin-embedded tissue (n=55). PR mRNA and protein expression correlated (P<0.0001). PR mRNA was a positive predictor for overall and progression-free survival (P=0.0005 and P<0.0001, respectively). Protein expression was also prognostic (P=0.015 and P=0.0011, respectively), whereas only PR mRNA retained its prognostic value on multivariate analysis (P=0.04). PR mRNA was still a positive prognostic marker among oestrogen receptor 1 (ESR1) mRNA-positive tumours (P=0.0007) and survival was best in patients with PR- and ESR1-positive phenotypes (P=0.0155 and P=0.0016, respectively). CONCLUSION: Expression of PR and ESR1 defines a subgroup of ovarian carcinomas with a favourable prognosis. PR and ESR1 mRNA expression analysis is a sensitive, quantitative and easy-to-perform high-throughput analytical tool for the identification of this subgroup and could be predictive in clinical trials focused on patients with potential benefit from hormonal treatment.
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Authors | Bruno V Sinn, Silvia Darb-Esfahani, Ralph M Wirtz, Jan Budczies, Jalid Sehouli, Radoslav Chekerov, Manfred Dietel, Carsten Denkert |
Journal | Histopathology
(Histopathology)
Vol. 59
Issue 5
Pg. 918-27
(Nov 2011)
ISSN: 1365-2559 [Electronic] England |
PMID | 22092403
(Publication Type: Journal Article)
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Copyright | © 2011 Blackwell Publishing Limited. |
Chemical References |
- Biomarkers, Tumor
- Estrogen Receptor alpha
- RNA, Messenger
- Receptors, Progesterone
- Formaldehyde
|
Topics |
- Biomarkers, Tumor
(analysis, genetics)
- Carcinoma
(genetics, metabolism, mortality)
- Disease-Free Survival
- Estrogen Receptor alpha
(biosynthesis, genetics)
- Female
- Formaldehyde
- High-Throughput Nucleotide Sequencing
- Humans
- Immunohistochemistry
- Kaplan-Meier Estimate
- Neoplasm Grading
- Neoplasm Staging
- Ovarian Neoplasms
(genetics, metabolism, mortality)
- Paraffin Embedding
- Prognosis
- RNA, Messenger
(analysis)
- Receptors, Progesterone
(biosynthesis, genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Tissue Fixation
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