Herbal formula CGX ameliorates LPS/D-galactosamine-induced hepatitis.

Abstract

CGX, a traditional herbal drug, has been prescribed for patients suffering from various liver diseases, including hepatitis B, alcoholic liver disease, and fatty liver. We investigated whether CGX has hepatoprotective effects against lipopolysaccharide/D-galactosamine (LPS/D-GalN)-induced acute liver injury and its underlying mechanism(s). Mice were administered CGX orally for 7 days prior to an injection of LPS (5 μg/kg)/D-GalN (700 mg/kg). Complete blood count, serum diagnostic markers, antioxidant activities, caspase activity, and histopathological examinations were conducted 8 h after the injection. To evaluate the immunological mechanism of CGX, serum TNF-α and IL-10 were investigated 1.5 h after LPS/D-GalN injection. CGX pretreatment (100, 200, and 400 mg/kg) inhibited the elevation of serum AST and ALT levels as well as histopathological alterations. Moreover, CGX pretreatment inhibited activation of caspase-3/7. CGX attenuated LPS/D-GalN-induced lipid peroxidation with concomitant improvement in total antioxidant activities (superoxide dismutase, catalase, and glutathione peroxidase). CGX elevated the antioxidant capacity of the liver in both the pathological and normal conditions. Furthermore, LPS/D-GalN-induced alterations of neutrophil and lymphocyte populations were ameliorated and serum TNF-α was decreased significantly by CGX. From these data we conclude that CGX protects the liver from LPS/D-GalN-induced hepatitis through antioxidant mechanisms as well as immune modulation.

• Authors: Jang Woo Shin, Jing Hua Wang, Hye Jung Park, Min Kyeong Choi, Hyeong Geug Kim, Chang Gue Son
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 49 Issue 6 Pg. 1329-34 (Jun 2011) ISSN: 1873-6351 [Electronic] England
• PMID: 21414374 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Ltd. All rights reserved.

Chemical References

• Antioxidants
• Immunologic Factors
• Lipopolysaccharides
• Plant Extracts
• Galactosamine
• Oxidoreductases
• Transaminases
• Caspases

Topics

• Animals
• Antioxidants (pharmacology)
• Caspases (metabolism)
• Chemical and Drug Induced Liver Injury (metabolism, pathology, prevention & control)
• Galactosamine (toxicity)
• Herbal Medicine
• Immunologic Factors (pharmacology)
• Lipid Peroxidation (drug effects)
• Lipopolysaccharides (toxicity)
• Liver (drug effects, metabolism)
• Lymphocytes (drug effects, pathology)
• Male
• Mice
• Mice, Inbred BALB C
• Neutrophils (drug effects, pathology)
• Oxidoreductases (metabolism)
• Plant Extracts (pharmacology)
• Transaminases (blood)