Genome-based expression profiles as a single standardized microarray platform for the diagnosis of experimental interstitial cystitis: an array of 75 genes model.

Abstract

INTRODUCTION AND HYPOTHESIS:

To investigate the molecular signature underlying experimental interstitial cystitis (IC) using cDNA microarray.

METHODS:

Microarray gene expression profiles are studied in bladder epithelium of C57BL/6 mice with ovalbumin or substance P-induced experimental IC versus Escherichia coli lipopolysaccharide-induced bacterial cystitis.

RESULTS:

Main findings are summarized as follows: firstly, a "75-gene" model was discovered to contain high expressions of bladder epithelium which feature in experimental IC. Secondly, glucose, lipid, nucleotide, xenobiotics, and amino acid metabolisms are involved in. Thirdly, T-cell-mediated immune and inflammatory responses are observed. Fourthly, Wnt, Tgf-beta, Mapk, and insulin growth factor receptor signaling pathways are also involved in. In addition, experimental IC leads to Ephrin- and Semaphorin-mediated axon guidance promoting parasympathetic inflammatory reflexes.

CONCLUSIONS:

Further characterization of human IC-induced gene expression profiles would enable the use of genome-based expression profiling for the therapeutic targets and diagnosis of IC.

• Authors: Ling-Hong Tseng, Ilene Chen, Ming-Yang Chen, Chyi-Long Lee, Tsia-Shu Lo, L Keith Lloyd
• Journal: International urogynecology journal and pelvic floor dysfunction (Int Urogynecol J Pelvic Floor Dysfunct) Vol. 20 Issue 5 Pg. 509-13 (May 2009) England
• PMID: 19153633 (Publication Type: Journal Article)

Chemical References

• Ephrins
• Lipopolysaccharides
• PAX2 Transcription Factor
• Pax2 protein, mouse
• Semaphorins
• Substance P
• Ovalbumin
• Nitric Oxide Synthase Type III
• Nos3 protein, mouse

Topics

• Animals
• Cluster Analysis
• Cystitis, Interstitial (chemically induced, diagnosis, genetics)
• Ephrins (genetics, metabolism)
• Escherichia coli
• Gene Expression Profiling
• Lipopolysaccharides
• Lymphocyte Activation (genetics)
• MAP Kinase Signaling System (genetics)
• Mice
• Mice, Inbred C57BL
• Nitric Oxide Synthase Type III (genetics)
• Oligonucleotide Array Sequence Analysis
• Ovalbumin
• PAX2 Transcription Factor (genetics)
• Semaphorins (genetics, metabolism)
• Substance P
• Transcriptome
• Up-Regulation
• Wnt Signaling Pathway (genetics)