[Effect of scalp acupuncture on inflammatory response in rats with acute cerebral ischemia-reperfusion injury].

Abstract

OBJECTIVE:

To explore the mechanism of scalp acupuncture (SA) in treating cerebral ischemia.

METHODS:

Sixty SD rats with middle cerebral artery occlusion (MCAO) were randomized into untreated group and SA-treated group, with another group of 10 SD rats without artery occlusion as sham-operated control. Neurological severity score (NSS), hemetoxylin and eosin (HE) staining and enzyme-linked immunosorbent assay were applied to observing the changes of neurofunctional defect, inflammatory infiltration in cerebral tissue and content of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta) and IL-10 at 24, 48 and 72 h after ischemia-reperfusion.

RESULTS:

There existed significant difference in NSS between SA-treated group and untreated group (P<0.01), especially at 72 h after ischemia-reperfusion. The HE staining results of ischemic cerebral tissue showed an apparent reduction of inflamed lesions in SA-treated group as compared with the untreated group (P<0.01), especially at 72 h after ischemia-reperfusion. The content of TNF-alpha and IL-1beta at each phase point after ischemia-reperfusion in SA-treated was decreased as compared with that in the untreated group, and there were differences between SA-treated group and untreated group at 72 h after ischemia-reperfusion (P<0.01). An apparent increase was observed in IL-10 between SA-treated group and untreated group at each phase point after ischemia-reperfusion, and there were significant differences between SA-treated group and untreated group at 48 and 72 h after ischemia-reperfusion (P<0.05, P<0.01).

CONCLUSION:

Scalp acupuncture can improve neurofunctional rehabilitation, suppress leukocyte infiltration, decrease the content of TNF-alpha and IL-1beta within a certain range and enhance IL-10 expression so as to suppress cytokines-mediated inflammatory reaction and attenuate cerebral ischemia-reperfusion injury.

• Authors: Hong-xing Zhang, Ling-guang Liu, Li Zhou, Hao Huang, Xuan Li, Min Yang
• Journal: Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine (Zhong Xi Yi Jie He Xue Bao) Vol. 5 Issue 6 Pg. 686-91 (Nov 2007) ISSN: 1672-1977 [Print] China
• PMID: 17997947 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-1beta
• Tumor Necrosis Factor-alpha
• Interleukin-10

Topics

• Acupuncture Therapy (methods)
• Animals
• Cerebral Infarction (metabolism, therapy)
• Electroacupuncture
• Female
• Inflammation (therapy)
• Interleukin-10 (metabolism)
• Interleukin-1beta (metabolism)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (therapy)
• Scalp
• Tumor Necrosis Factor-alpha (metabolism)