Abstract | BACKGROUND:
DNA of cutaneous human papillomavirus (HPV) types is frequently found in nonmelanoma skin cancer, and their E6 and E7 proteins can have transforming properties. OBJECTIVES: To assess the biological activity of HPV types found in tumour tissues we examined HPV E6/E7 RNA expression and the antibody response to E6, E7 and L1 proteins. METHODS: RESULTS: HPV DNA was detected in 25 of 31 tissue samples, indicating eight single and 17 multiple HPV infections. E6/E7 transcripts of HPV 8, 9 and 15 were found in low copy numbers in one SCC and three AKs, but not in normal skin or Vv. All four patients examined showed antibodies to cutaneous HPV antigens, but the antibody response did not correlate with E6/E7 expression detected in the tumour. CONCLUSIONS: Transcriptional activity of the E6/E7 oncogenes in AK and SCC suggests an active role of HPV in the lesion.
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Authors | C Dang, A Koehler, T Forschner, P Sehr, K Michael, M Pawlita, E Stockfleth, I Nindl |
Journal | The British journal of dermatology
(Br J Dermatol)
Vol. 155
Issue 1
Pg. 129-36
(Jul 2006)
ISSN: 0007-0963 [Print] England |
PMID | 16792764
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Viral
- Capsid Proteins
- DNA, Viral
- DNA-Binding Proteins
- E6 protein, Human papillomavirus type 8
- E6 protein, human papillomavirus type 5
- HPV L1 protein, Human papillomavirus
- Oncogene Proteins, Viral
- Papillomavirus E7 Proteins
- RNA, Viral
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Topics |
- Antibodies, Viral
(blood)
- Capsid Proteins
(genetics)
- Carcinoma, Squamous Cell
(immunology, virology)
- DNA, Viral
(analysis)
- DNA-Binding Proteins
(genetics)
- Humans
- Immunocompromised Host
- Oncogene Proteins, Viral
(genetics)
- Organ Transplantation
- Papillomavirus E7 Proteins
(genetics, immunology)
- RNA, Viral
(analysis)
- Reverse Transcriptase Polymerase Chain Reaction
- Skin
(immunology, pathology, virology)
- Skin Neoplasms
(immunology, virology)
- Viral Load
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