Abstract | PURPOSE: MATERIALS AND METHODS: Genotypes of the leucine (Leu) to proline (Pro) polymorphism at codon 198 of GPX1, the alanine (Ala) to Valine (Val) polymorphism in exon 2 and the isoleucine to threonine polymorphism at codon 56 of MnSOD were determined by a polymerase chain reaction-restriction fragment length polymorphism technique in 213 patients and 209 normal controls. RESULTS: There was a significant difference in GPX1 genotype frequency between the case and control groups (p = 0.001). The adjusted OR for bladder cancer was 2.63 for the Pro/Leu genotype compared with the Pro/Pro genotype (95% CI 1.45 to 4.75, p = 0.001). Compared with the Pro/Pro genotype the Pro/Leu genotype was significantly associated with advanced tumor stage (Ta-1 vs T2-4, OR 2.58, 95% CI 1.07 to 6.18, p = 0.034) but not with tumor grade. Analysis of the MnSOD polymorphism provided no significant results. However, in men with at least 1 Ala MnSOD allele the risk associated with the Pro/Leu GPX1 genotype increased up to 6.31 (95% CI 1.28 to 31.24, p = 0.024). CONCLUSIONS: The GPX1 Pro/Leu genotype may significantly increase the risk of bladder cancer and the increased risk may be modified by the Ala-9Val MnSOD polymorphism. The GPX1 genotype may further affect the disease status of bladder cancer.
|
Authors | Yasushi Ichimura, Tomonori Habuchi, Norihiko Tsuchiya, Lizhong Wang, Chikara Oyama, Kazunari Sato, Hiroyuki Nishiyama, Osamu Ogawa, Tetsuro Kato |
Journal | The Journal of urology
(J Urol)
Vol. 172
Issue 2
Pg. 728-32
(Aug 2004)
ISSN: 0022-5347 [Print] United States |
PMID | 15247771
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Proline
- Glutathione Peroxidase
- Superoxide Dismutase
- Leucine
- Valine
- Alanine
- Glutathione Peroxidase GPX1
- GPX1 protein, human
|
Topics |
- Aged
- Alanine
(genetics)
- Asian People
(genetics)
- Carcinoma, Transitional Cell
(genetics)
- Female
- Genetic Predisposition to Disease
- Genotype
- Glutathione Peroxidase
(genetics)
- Humans
- Leucine
(genetics)
- Logistic Models
- Male
- Middle Aged
- Odds Ratio
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Proline
(genetics)
- Superoxide Dismutase
(genetics)
- Urinary Bladder Neoplasms
(genetics)
- Valine
(genetics)
- Glutathione Peroxidase GPX1
|