Abstract | BACKGROUND: METHODS: A total of 38 specimens which had been collected from 36 patients and fulfilled the criteria for tissues described above were used in this study. Two different primer pairs targeting the gene encoding for 16S ribosomal RNA (common to all mycobacteria) and the insertion sequence IS6110 (specific for M. tuberculosis complex) were used in the PCR assays. The clinical characteristics, histopathologic findings, and culture results of the patients were also analyzed. RESULTS: Four specimens were excluded from the analysis due to the lack of internal control testing. Of the remaining 34 specimens, 22 were PCR positive for the 16S rRNA gene. Among them, 18 specimens were PCR positive for both the 16S rRNA gene and IS6110. Cutaneous tuberculosis could be diagnosed in these 18 cases (56.2%). Out of the 18 cases, there were 8 women and 10 men. The age range was 15-77 years (mean: 44.2 years). After reviewing their clinical presentation, 11 cases were considered as tuberculosis verrucosa cutis, 6 cases as lupus vulgaris, and 1 case as erythema induratum. The remaining 4 cases (12.5%) positive only for 16S rRNA gene were considered as possible atypical mycobacteria infection. CONCLUSIONS:
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Authors | Pa-Fan Hsiao, Chin-Yuan Tzen, Hsiu-Chin Chen, Hsin-Yi Su |
Journal | International journal of dermatology
(Int J Dermatol)
Vol. 42
Issue 4
Pg. 281-6
(Apr 2003)
ISSN: 0011-9059 [Print] England |
PMID | 12694493
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Adolescent
- Adult
- Aged
- Female
- Granuloma
(microbiology)
- Humans
- In Vitro Techniques
- Male
- Middle Aged
- Mycobacterium Infections, Nontuberculous
(diagnosis, microbiology)
- Mycobacterium tuberculosis
(isolation & purification)
- Polymerase Chain Reaction
(methods)
- Staining and Labeling
(methods)
- Tuberculosis, Cutaneous
(diagnosis, microbiology)
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