Abstract | BACKGROUND AND AIMS: Ectopic production of prolactin has been reported for several tumors, and elevated prolactin plasma levels have been detected in colorectal cancer patients. However, the role of prolactin in colorectal cancer remains unclear. We therefore compared expression patterns of prolactin and its receptor in normal and neoplastic colonic mucosa of the same patient with noncancer controls and determined mitogenic effects in vitro. MATERIALS AND METHODS:
mRNA and protein expression was analyzed by semiquantitative RT-PCR and western blotting. Localization of ligand and receptor in tissues was investigated by immunohistochemistry. Mitogenic effects of prolactin on colorectal cancer cell lines (Caco-2, HT-29, LoVo) were assayed by [(3)H] thymidine incorporation. RESULTS:
mRNA expression of prolactin was detected in 13% of normal and 27% of cancer specimens, with the highest levels seen in moderately differentiated tumors. Receptor mRNA was amplified from the majority of normal (96%) and cancer (94%) samples with an overexpression seen in tumor tissues. Protein expression of prolactin was detected in cancer tissues only, with the highest levels seen in moderately differentiated tumors. Receptor protein expression was correlated with the RT-PCR data, showing up to fourfold overexpression in tumor tissues. Staining for both ligand and receptor was observed in epithelial cells. DNA synthesis was significantly stimulated by prolactin in all cell lines reaching 167-197% of unstimulated controls. CONCLUSION:
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Authors | Jan-Michel Otte, Claudia Otte, Stefanie Beckedorf, Frank Schmitz, Barbara K Vonderhaar, Ulrich R Fölsch, Sievert Kloehn, Karl-Heinz Herzig, Heiner Mönig |
Journal | International journal of colorectal disease
(Int J Colorectal Dis)
Vol. 18
Issue 1
Pg. 86-94
(Jan 2003)
ISSN: 0179-1958 [Print] Germany |
PMID | 12458387
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Actins
- Biomarkers, Tumor
- RNA, Messenger
- Receptors, Prolactin
- Prolactin
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Topics |
- Actins
(metabolism)
- Adenocarcinoma, Mucinous
(genetics, metabolism)
- Adult
- Aged
- Aged, 80 and over
- Biomarkers, Tumor
(metabolism)
- Blotting, Western
- Carcinoma, Signet Ring Cell
(genetics, metabolism)
- Colorectal Neoplasms
(genetics, metabolism)
- Female
- Humans
- Immunohistochemistry
- Intestinal Mucosa
(cytology, metabolism)
- Male
- Middle Aged
- Neoplasm Staging
- Prolactin
(biosynthesis, genetics)
- RNA, Messenger
(metabolism)
- Receptors, Prolactin
(biosynthesis, genetics)
- Statistics as Topic
- Tumor Cells, Cultured
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