Expression of the chemokines MCP-1/JE and cytokine-induced neutrophil chemoattractant in early acute pancreatitis.

Abstract

INTRODUCTION:

Inflammatory mediators play a critical role in acute pancreatitis. The precise role played by members of the chemokine family remains unclear.

AIMS:

To investigate the expression of the CC chemokine monocyte chemotactic protein (MCP)-1/JE and the CXC chemokine cytokine-induced neutrophil chemoattractant (CINC) in early acute pancreatitis.

METHODOLOGY:

Pancreatitis was induced in rats, either by intraperitoneal injection of cerulein or by infusion of 5% sodium taurocholate into the pancreatic duct. Expression of MCP-1/JE and CINC in pancreas and plasma was determined by immunohistochemistry, enzyme-linked immunosorbent assay (ELISA), Northern analysis, and quantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR).

RESULTS:

Following induction of acute pancreatitis, MCP-1/JE and CINC immunoreactivity was seen in acinar cells. Infiltrating neutrophils were strongly immunolabeled with an anti-MCP-1/JE antibody, whereas macrophages reacted strongly with an antibody to CINC. Northern analysis and quantitative real-time RT-PCR demonstrated upregulation of MCP-1/JE and CINC mRNA levels in pancreatic tissue. Plasma MCP-1 levels were significantly increased after 6 hours in the cerulein hyperstimulation model (2,444 +/- 93 microg/mL versus control, 1,853 +/- 262 microg/mL; < 0.05). Plasma CINC levels were significantly increased after 6 hours in the cerulein hyperstimulation model (1,680 +/- 134 microg/mL versus control, 725 +/- 128 microg/mL; < 0.005) and after 3 hours in the bile salt infusion model (6,663 +/- 1,405 microg/mL versus control, 2,339 +/- 800 microg/mL; < 0.05).

CONCLUSION:

CINC and MCP-1/JE may be early mediators of the inflammatory response in acute pancreatitis.

• Authors: Mark Brady, Madhav Bhatia, Stephen Christmas, Mark T Boyd, John P Neoptolemos, John Slavin
• Journal: Pancreas (Pancreas) Vol. 25 Issue 3 Pg. 260-9 (Oct 2002) ISSN: 1536-4828 [Electronic] United States
• PMID: 12370537 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Chemokine CCL2
• Chemokines
• Chemokines, CXC
• Chemotactic Factors
• Intercellular Signaling Peptides and Proteins
• RNA, Messenger

Topics

• Acute Disease
• Animals
• Chemokine CCL2 (biosynthesis, blood, genetics)
• Chemokines (biosynthesis, blood, genetics)
• Chemokines, CXC
• Chemotactic Factors (biosynthesis, blood, genetics)
• Enzyme-Linked Immunosorbent Assay
• Immunohistochemistry
• Intercellular Signaling Peptides and Proteins (biosynthesis, blood, genetics)
• Male
• Pancreatitis (genetics, immunology, pathology)
• RNA, Messenger (biosynthesis)
• Rats
• Rats, Wistar
• Time Factors
• Transcription, Genetic