Catecholamines play a role in the production of interleukin-6 and interleukin-1alpha in unburned skin after burn injury in mice.

Abstract

OBJECTIVE:

To investigate the effects of catecholamines on the production of interleukin (IL)-6 and IL-1alpha in unburned skin after a burn injury.

DESIGN:

Randomized, controlled study.

SETTING:

Animal laboratory.

SUBJECTS:

Anesthetized female C57BL/6 mice.

INTERVENTIONS:

A full-thickness burn injury on 20% of total body surface area was produced on the clipped dorsum, which was followed by resuscitation with lactated Ringer's solution (0.1 mL/g). Phentolamine mesylate (10 mg/kg) or propranolol hydrochloride (10 mg/kg) was administered intraperitoneally before the burn injury.

MEASUREMENTS AND MAIN RESULTS:

Epinephrine and norepinephrine increased the production of IL-6 but not of IL-1alpha in normal abdominal skin, and these increases were reversed by a beta-blocker (propranolol hydrochloride) but not an alpha-blocker (phentolamine mesylate). A 20% full-thickness burn injury of the skin increased the plasma concentration of epinephrine 30 mins after the injury in female C57BL/6 mice, but not norepinephrine or dopamine. The burn also increased IL-6 production in unburned abdominal skin. The administration of a beta-blocker before a 20% burn injury suppressed the increased IL-6 production in unburned abdominal skin as determined by both protein level and mRNA expression. Pretreatment with the alpha-blocker was less effective.

CONCLUSIONS:

We propose that the neuroendocrine system activated by a 20% burn injury participates in the increased IL-6 production in the unburned skin.

• Authors: M Kawakami, J He, T Sakamoto, Y Okada
• Journal: Critical care medicine (Crit Care Med) Vol. 29 Issue 4 Pg. 796-801 (Apr 2001) ISSN: 0090-3493 [Print] United States
• PMID: 11373471 (Publication Type: Journal Article)

Chemical References

• Adrenergic alpha-Antagonists
• Adrenergic beta-Antagonists
• Interleukin-1
• Interleukin-6
• Propranolol
• Norepinephrine
• Epinephrine
• Phentolamine

Topics

• Adrenergic alpha-Antagonists (pharmacology)
• Adrenergic beta-Antagonists (pharmacology)
• Animals
• Burns (metabolism)
• Chromatography, High Pressure Liquid
• Epinephrine (blood, physiology)
• Female
• Interleukin-1 (biosynthesis)
• Interleukin-6 (biosynthesis)
• Mice
• Mice, Inbred C57BL
• Norepinephrine (blood, physiology)
• Phentolamine (pharmacology)
• Propranolol (pharmacology)
• Reverse Transcriptase Polymerase Chain Reaction
• Skin (drug effects)