Abstract | BACKGROUND: METHODS: The genomic DNA of infant leukemia, childhood neuroblastoma, and control individuals was amplified by polymerase chain reaction (PCR). Patients who had heterozygous genotype were selected as informative cases using Apa I polymorphism in exon 9 of the IGF-2 gene. Total RNA was isolated from informative cases, followed by cDNA synthesis. cDNA was amplified by PCR, and direct sequence was performed for determining allele specific transcription. RESULTS: CONCLUSIONS: The current study revealed that the imprinting status of IGF-2 was generally maintained in infant leukemia and confirmed that it was maintained in childhood neuroblastoma. The results suggest that LOI of IGF-2 does not play a major role in the carcinogenesis of these diseases or in rapid physical growth of the patients.
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Authors | H Hattori, A Matsuzaki, A Suminoe, K Ihara, M Eguchi, T Tajiri, S Suita, E Ishii, T Hara |
Journal | Cancer
(Cancer)
Vol. 88
Issue 10
Pg. 2372-7
(May 15 2000)
ISSN: 0008-543X [Print] United States |
PMID | 10820361
(Publication Type: Journal Article)
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Chemical References |
- Insulin-Like Growth Factor II
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Topics |
- Female
- Genomic Imprinting
- Humans
- Infant
- Insulin-Like Growth Factor II
(genetics)
- Leukemia
(genetics)
- Male
- Neuroblastoma
(genetics)
- Polymerase Chain Reaction
- Polymorphism, Genetic
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