Abstract | PURPOSE: We previously reported a novel cytoskeletal protein with a myosin-like domain which is localized in the ciliary rootlet and basal body of connecting cilium of photoreceptor and hence we named it ' myocilin'. It was soon realized that myocilin is identical to a protein called TIGR (trabecular meshwork inducible glucocorticoid response protein) which was found to be responsible for the pathogenesis of juvenile open angle glaucoma. In this study, we employed in situ RNA hybridization to examine the myocilin (MYOC)/ TIGR gene expression in the trabecular meshworks of glaucomatous and nonglaucomatous eyes. METHODS: RESULTS: In all cases, hybridization signals were detected primarily in the trabecular meshwork cells and secondarily in the fibroblast-like cells of corneoscleral wall. CONCLUSIONS:
Myocilin gene is expressed clearly in the trabecular meshwork cells of both glaucomatous and nonglaucomatous eyes.
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Authors | H Takahashi, S Noda, Y Mashima, R Kubota, Y Ohtake, T Tanino, J Kudoh, S Minoshima, Y Oguchi, N Shimizu |
Journal | Current eye research
(Curr Eye Res)
Vol. 20
Issue 2
Pg. 81-4
(Feb 2000)
ISSN: 0271-3683 [Print] England |
PMID | 10617907
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cytoskeletal Proteins
- Eye Proteins
- Glycoproteins
- RNA, Messenger
- trabecular meshwork-induced glucocorticoid response protein
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Topics |
- Aged
- Cytoskeletal Proteins
- Eye
(metabolism, pathology)
- Eye Proteins
(genetics)
- Female
- Gene Expression
- Glaucoma
(genetics)
- Glycoproteins
(genetics)
- Humans
- In Situ Hybridization
- Male
- Middle Aged
- RNA, Messenger
(genetics)
- Trabecular Meshwork
(metabolism)
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