The role of guanine nucleotide binding proteins in hamsters with myocardial hypertrophy.

Abstract

To clarify the role of the guanine nucleotide binding proteins (G-proteins) in the pathogenesis of myocardial hypertrophy, we investigated the alterations in myocardial G proteins in 20-week-old F1b hamsters with pressure overload induced for 3 days (3-day AS), 7 days (7-day AS) and 14 days (14-day AS) by the stenosis of the abdominal aorta, and in 4- and 20 week-old BIO 14.6 Syrian hamsters (4-wk and 20-wk BIO) with genetic myocardial hypertrophy. The hearts of 7-day AS, 14-day AS and 20-wk BIO with left ventricular hypertrophy exhibited a decrease in the mRNA levels detected by Northern blot analysis and protein levels of G protein detected by Western blot analysis as compared with sham-operated and age-matched F1b hearts. The function of Gs or Gi showed a concomitant reduction in both models of myocardial hypertrophy. The hearts of 3-day AS and 4-wk BIO without myocardial hypertrophy showed no changes in G proteins as compared with sham-operated and age-matched F1b hearts. These results suggest that a decrease in G proteins is not involved in the pathogenesis of myocardial hypertrophy, but that myocardial hypertrophy reduced the G proteins.

• Authors: K Nakamura, M Ohyanagi, J Shibuya, J Yamamoto, T Iwasaki
• Journal: Journal of receptor and signal transduction research (J Recept Signal Transduct Res) 1996 May-Jul Vol. 16 Issue 3-4 Pg. 225-42 ISSN: 1079-9893 [Print] England
• PMID: 8897313 (Publication Type: Journal Article)

Chemical References

• RNA, Messenger
• GTP-Binding Proteins
• Adenylyl Cyclases

Topics

• Adenylyl Cyclases (metabolism)
• Animals
• Blotting, Northern
• Blotting, Western
• Body Weight
• Cardiomegaly (etiology, metabolism)
• Cricetinae
• GTP-Binding Proteins (genetics, physiology)
• Male
• Mesocricetus
• Organ Size
• RNA, Messenger (analysis)