Effects of albiflorin on oxidative stress and inflammatory responses in rats with acute spinal cord injury.

Abstract

INTRODUCTION:

Oxidative stress and inflammatory responses are often the predominant detrimental factors associated with spinal cord injury (SCI). This study investigates the potential therapeutic effects of albiflorin (AF) on alleviating inflammation and oxidative stress in the rat model with SCI.

METHODS:

Initially, the behavior of SCI-induced rats is examined by Basso-Beattie-Bresnahan score and the inclined plane examination. Then, the immunohistochemical staining of inflammasome-related protein (for instance, NACHT, LRR, and PYD domains-containing protein 3, NLRP3) is performed in combination with enzyme-linked immunosorbent assay (ELISA) of corresponding proinflammatory factors to assess the immunomodulatory effects of AF. Further, the markers involved in oxidative stress are examined by ELISA and western blot analysis analyses.

RESULTS:

These findings indicated that AF could alleviate motor dysfunction and the loss of neuron cells in SCI-induced rats. Mechanistically, AF could attenuate the inflammatory responses by reducing oxidative stress and activating nuclear erythroid-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway in SCI rats. Depleting the antioxidant capacity by inhibiting glutathione biosynthesis could counteract the anti-inflammatory activity of AF in SCI rats.

CONCLUSIONS:

Together, our data suggested that AF could serve as a potential therapeutic agent against the aggravation of SCI in rats.

• Authors: Pengfei Fang, Yi Wang, Fengqi Sun, Haisheng Lin, Xindong Zhang
• Journal: Immunity, inflammation and disease (Immun Inflamm Dis) Vol. 11 Issue 9 Pg. e1015 (09 2023) ISSN: 2050-4527 [Electronic] England
• PMID: 37773716 (Publication Type: Journal Article)
• Copyright: © 2023 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd.

Chemical References

• albiflorin
• Bridged-Ring Compounds

Topics

• Rats
• Animals
• Rats, Sprague-Dawley
• Spinal Cord Injuries (drug therapy, metabolism)
• Oxidative Stress
• Bridged-Ring Compounds (pharmacology, therapeutic use)