Alzheimer's disease (AD) is a common chronic progressive
neurodegenerative disorder, and curative treatment has not been developed. The objective of this study was to investigate the potential effects of
hydralazine (Hyd, a
hypertension treatment
drug) on the development process of AD and its mechanisms. We treated 6-month-old male APP/PS1 mice with Hyd for 5 weeks, measured changes in behavior and pathological status, and analyzed differences in gene expression by
RNA sequencing. The results demonstrated that Hyd improved cognitive deficits and decreased
amyloid beta protein deposition in the cortex and hippocampus, while
RNA sequencing analysis suggested that the regulation of
neuroinflammation and energy metabolism might play pivotal roles for Hyd's beneficial effects. Therefore, we further investigated inflammatory response, redox state, and mitochondrial function, as well as the expression of
toll-like receptor 4 (TLR4)/
nuclear factor Kappa B (NF-κB)-dependent
neuroinflammation gene and nuclear factor erythroid 2-related factor 2 (Nrf2)-mediated
antioxidant gene in AD mice. The results showed that Hyd reduced the damage of
neuroinflammation and oxidative stress, improved
mitochondrial dysfunction, downregulated pro-
inflammation gene expression, and upregulated
antioxidant gene expression. The results in
lipopolysaccharide (LPS)-induced BV2 cell model demonstrated that Hyd suppressed pro-inflammatory response via TLR4/NF-κB signaling pathway. In addition, by silencing the Nrf2 gene expression, it was found that Hyd can reduce LPS-induced
reactive oxygen species production by activating the Nrf2 signaling pathway. Therefore, administration of Hyd in the early stage of AD might be beneficial in delaying the pathological development of AD via inhibiting
neuroinflammation and oxidative stress.