Aspergillus fumigatus causes
aspergillosis with high morbidity and mortality in the duck industry. As a vital
virulence factor produced by A. fumigatus,
gliotoxin (GT) is widely present in food and feed, threatening duck industry and human health.
Quercetin is a
polyphenol flavonoid compound from natural plants with anti-inflammatory and
antioxidant functions. However, the effects of
quercetin on ducklings with GT
poisoning are unknown. The model of ducklings with GT
poisoning was established, and the protective effects and molecular mechanisms of
quercetin on ducklings with GT
poisoning were investigated. Ducklings were divided into control, GT, and
quercetin groups. A model of GT (2.5 mg/kg)
poisoning in ducklings was successfully established.
Quercetin protected GT-induced liver and kidney functions and alleviated GT-induced alveolar wall thickening in lungs, cell fragmentation, and inflammatory cell infiltration in liver and kidney.
Quercetin decreased
malondialdehyde (MDA) and increased
superoxide dismutase (SOD) and
catalase (CAT) after GT treatment.
Quercetin significantly reduced GT-induced
mRNA expression levels of inflammatory factors. Furthermore,
quercetin increased GT-reduced heterophil extracellular traps (HETs) in serum. These results indicated that
quercetin protected ducklings against GT
poisoning by inhibiting oxidative stress,
inflammation and increasing HETs release, which confirms the potential applicability of
quercetin in treating GT-induced duckling
poisoning.