Tanshinone Protects against Spinal Cord Ischemia-Reperfusion Injury by Inhibiting JNK Activity.

Abstract

Spinal cord reperfusion injury as a secondary damage after primary spinal cord injury is an important factor causing nerve cell damage. In this study, we aim to investigate the effects and mechanisms of tanshinone (TAE) in the rabbit spinal cord during ischemia-reperfusion. New Zealand white rabbits were randomly divided into 3 groups: sham-operated group (5 rabbits), ischemia-reperfusion group (0.9% TAE was administered intraperitoneally 30 min before ischemia, and 4 groups of 5 rabbits each according to different time periods of reperfusion: group A reperfused for 0.5 h, group B reperfused for 2 h, group C reperfused for 8 h, and group D reperfused for 24 h), and TAE group (an ischemia-reperfused for 24 h). Group A was reperfused for 0.5 h, group B for 2 h, group C for 8 h, group D for 24 h, and group TAE (TAE was applied 30 min before ischemia reperfusion, grouped as ischemia-reperfusion group). The expression of JNK (c-Jun NH2-terminal Kinase) and phosphorylation-JNK (p-JNK) in spinal cord tissues of each group were detected by Western blot. Light and electron microscopy showed that early apoptosis started in group B in the ischemia-reperfusion group, while early apoptosis appeared only in group D in the tanshinone intervention group. Western blot showed that p-JNK expression started in group B in the ischemia-reperfusion group and gradually increased with the prolongation of ischemia time, while p-JNK expression only increased in group D in the tanshinone intervention group. In the tanshinone intervention group, p-JNK was activated only in group D and its activity was less than that in the ischemia-reperfusion group; the protein expression of JNK did not change significantly in both groups. Spinal cord ischemia-reperfusion can cause spinal cord injury by activating the signaling molecule JNK (MRPKs family), and early tanshinone intervention can partially inhibit this injury. Our finding provides a new idea and theoretical basis for clinical treatment of spinal cord ischemia-reperfusion injury.

• Authors: Peng Zhang, Qingyu Zhang, Xiaoyang Liu, Tao Li, Shuai Wu, Yi Li, Zhensong Jiang
• Journal: Computational intelligence and neuroscience (Comput Intell Neurosci) Vol. 2022 Pg. 7619797 ( 2022) ISSN: 1687-5273 [Electronic] United States
• PMID: 35602615 (Publication Type: Journal Article, Retracted Publication)
• Copyright: Copyright © 2022 Peng Zhang et al.

Chemical References

• Abietanes
• tanshinone

Topics

• Abietanes
• Animals
• Ischemia (complications, metabolism)
• Phosphorylation
• Rabbits
• Reperfusion Injury (drug therapy, metabolism, prevention & control)
• Spinal Cord
• Spinal Cord Injuries (complications, drug therapy, metabolism)
• Spinal Cord Ischemia (drug therapy, etiology, metabolism)