Lipid mediators derived from
arachidonic acid (AA) are implicated with the occurrence of
inflammation and oxidative stress. The current knowledge of AA metabolism focuses on searching for the therapeutic strategy to subvert affected AA metabolism. The aim of our study was to evaluate the potential protective effect of chronic α-
lipoic acid (α-LA) supplementation on myocardial
inflammation state and oxidative stress in
obesity-related cardiovascular dysfunction. The experiment was carried out on male Wistar rats receiving a standard or a high-fat diets with intragastric α-LA administration for 8 weeks. Plasma and myocardial AA concentrations were determined using the gas-liquid chromatography (GLC). The Western blot technique was used to examine the expression of
proteins from the inflammatory pathway. The content of selected
cytokines, inflammatory mediators, and oxidative stress indicators was detected by the ELISA, colorimetric, and multiplex assay kits. Our results revealed that α-LA caused a notable reduction in AA content, mainly in the
phospholipid fraction with a simultaneous diminishment in the synthesis of pro-inflammatory mediators, i.e.,
prostaglandin E2,
leukotrienes B4 and C4 by decreasing the expression of COX-2 and 5-LOX. α-LA also augmented the level of antioxidative SOD2 and GSH and decreased the level of lipid peroxidation products, which improved oxidative system impairment in the left ventricle tissue. The data clearly showed that α-
lipoic acid has a significant role in
inflammation and oxidative stress development ameliorating the risk of cardiac
obesity induced by high-fat feeding.