Abstract | OBJECTIVE: To observe the effect of acupuncture at "Yanglingquan" (GB34) and "Baihui" (GV20) on Na+/K+- ATPase, excitatory amino acid transporters (EAATs) and glutamate (Glu) in hippocampus of post- stroke spasticity rats, so as to explore the central mechanism in anti-spasticity. METHODS: In a total of 48 healthy SD rats, 12 rats were randomly selected to be included into sham operation group, and the remaining rats were used to make a middle cerebral artery occlusion (MCAO) model using a suture method. On the 3rd day after modeling, MCAO limb spasticity rats were screened by neurological deficit symptoms and muscle tension scores, and randomly divided into the model, GB34 (Hui- puncture at GB34) and GB34+GV20 (Hui- puncture at GB34 and horizontal insertion at GV20) groups (n=12 rats in each group), and the treatment was lasted for 7 conse-cutive days. The neurological symptoms and muscle tension score were observed with the Zea Longa score and modified Ashworth scale (MAS). The levels of Glu, EAAT1 (GLAST) and EAAT2 (GLT-1) in the ischemic area of cerebral hippocampus were detected by ELISA, the expression of Na+/K+- ATPase α1 (ATP1α1) was detected by Western blot, the expression of ATP1α1 mRNA was detected by real-time PCR, and the expression of GLAST, GLT-1 and ATP1α1 was detected by immunofluorescence. RESULTS: After modeling, Zea Longa score and MAS score were increased (P<0.01), the level of Glu in the ischemic area of cerebral hippocampus was increased (P<0.01), while the expression levels of GLAST, GLT-1, ATP1α1 protein and mRNA were all decreased (P<0.01) in the model group relevant to the sham operation group. After 7 days' treatment, all the increased and decreased levels of the indexes mentioned above were reversed in the two acupuncture groups relevant to the model group (P<0.01, P<0.05), and the effects of acupuncture at GB34+GV20 were obviously superior to that of acupuncture at GB34 (P<0.05, P<0.01). CONCLUSION: Acupuncture can alleviate post- stroke spasticity effectively, which may be related to its effect in up-regulating the expressions of Na+/K+- ATPase and EAATs in hippocampus. The anti- spastic effect of acupuncture at GB34+GV20 is superior to GB34 alone.
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Authors | Xu Qian, Liang-Xiao Ma, Jie-Dan Mu, Zhou Zhang, Tian-Yi Sun, Wen-Yan Yu, Yuan Tian, Yi-Dan Zhang |
Journal | Zhen ci yan jiu = Acupuncture research
(Zhen Ci Yan Jiu)
Vol. 47
Issue 4
Pg. 283-9
(Apr 25 2022)
ISSN: 1000-0607 [Print] China |
PMID | 35486007
(Publication Type: Journal Article)
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Chemical References |
- RNA, Messenger
- Glutamic Acid
- Adenosine Triphosphatases
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Topics |
- Acupuncture Points
- Acupuncture Therapy
(methods)
- Adenosine Triphosphatases
- Animals
- Glutamic Acid
- Infarction, Middle Cerebral Artery
- Muscle Spasticity
(genetics, therapy)
- RNA, Messenger
- Rats
- Rats, Sprague-Dawley
- Stroke
(complications, genetics, therapy)
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