Abstract | BACKGROUND & AIMS: Liver sinusoidal endothelial cells (LSECs) are ideally situated to sense stiffness and generate angiocrine programs that potentially regulate liver fibrosis and portal hypertension. We explored how specific focal adhesion (FA) proteins parlay LSEC mechanotransduction into stiffness-induced angiocrine signaling in vitro and in vivo. METHODS: Primary human and murine LSECs were placed on gels with incremental stiffness (0.2 kPa vs. 32 kPa). Cell response was studied by FA isolation, actin polymerization assay, RNA-sequencing and electron microscopy. Glycolysis was assessed using radioactive tracers. Epigenetic regulation of stiffness-induced genes was analyzed by chromatin-immunoprecipitation (ChIP) analysis of histone activation marks, ChIP sequencing and circularized chromosome conformation capture (4C). Mice with LSEC-selective deletion of glycolytic enzymes (Hk2fl/fl/Cdh5cre-ERT2) or treatment with the glycolysis inhibitor 3PO were studied in portal hypertension (partial ligation of the inferior vena cava, pIVCL) and early liver fibrosis (CCl4) models. RESULTS: Glycolytic enzymes, particularly phosphofructokinase 1 isoform P (PFKP), are enriched in isolated FAs from LSECs on gels with incremental stiffness. Stiffness resulted in PFKP recruitment to FAs, which paralleled an increase in glycolysis. Glycolysis was associated with expansion of actin dynamics and was attenuated by inhibition of integrin β1. Inhibition of glycolysis attenuated a stiffness-induced CXCL1-dominant angiocrine program. Mechanistically, glycolysis promoted CXCL1 expression through nuclear pore changes and increases in NF-kB translocation. Biochemically, this CXCL1 expression was mediated through spatial re-organization of nuclear chromatin resulting in formation of super-enhancers, histone acetylation and NF-kB interaction with the CXCL1 promoter. Hk2fl/fl/Cdh5cre-ERT2 mice showed attenuated neutrophil infiltration and portal hypertension after pIVCL. 3PO treatment attenuated liver fibrosis in a CCl4 model. CONCLUSION: Glycolytic enzymes are involved in stiffness-induced angiocrine signaling in LSECs and represent druggable targets in early liver disease. LAY SUMMARY:
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Authors | Thomas Greuter, Usman Yaqoob, Can Gan, Nidhi Jalan-Sakrikar, Enis Kostallari, Jianwen Lu, Jinhang Gao, Liankang Sun, Mengfei Liu, Tejasav S Sehrawat, Samar H Ibrahim, Kunimaro Furuta, Katerina Nozickova, Bing Q Huang, Bin Gao, Michael Simons, Sheng Cao, Vijay H Shah |
Journal | Journal of hepatology
(J Hepatol)
Vol. 77
Issue 3
Pg. 723-734
(09 2022)
ISSN: 1600-0641 [Electronic] Netherlands |
PMID | 35421427
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2022 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved. |
Chemical References |
- Actins
- CXCL1 protein, human
- Chemokine CXCL1
- Chromatin
- Histones
- NF-kappa B
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Topics |
- Actins
(metabolism)
- Animals
- Chemokine CXCL1
(metabolism)
- Chromatin
(metabolism)
- Endothelial Cells
(metabolism)
- Epigenesis, Genetic
- Glycolysis
- Histones
(metabolism)
- Humans
- Hypertension, Portal
(metabolism)
- Liver
(pathology)
- Liver Cirrhosis
(pathology)
- Mechanotransduction, Cellular
- Mice
- NF-kappa B
(metabolism)
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