Chronic obstructive pulmonary disease (
COPD) is a prevalent respiratory disease. Aiming at assessing the effect of total
saponins from American ginseng on
COPD, both the chemical composition and anti-
COPD activity of total
saponins from wild-simulated American ginseng (TSW) and field-grown American ginseng (
TSF) were investigated in this study. Firstly, a HPLC-ELSD chromatographic method was established to simultaneously determine the contents of 22
saponins in TSW and
TSF. Secondly, CS-induced
COPD mouse model was established to evaluate the activity of TSW and
TSF. The results indicated that both TSW and
TSF had the protective effect against
COPD by alleviating oxidative stress and inflammatory response. TSW showed a stronger effect than
TSF. Thirdly, an integrated approach involving metabolomics and network pharmacology was used to construct the "
biomarker-reaction-
enzyme-target" correlation network aiming at further exploring the observed effects. As the results, 15
biomarkers, 9 targets and 5 pathways were identified to play vital roles in the treatment of TSW and
TSF on
COPD. Fourthly, based on network pharmacology and the CS-stimulated A549 cell model,
ginsenoside Rgl, Rc,
oleanolic acid,
notoginsenoside R1, Fe, silphioside B were certified to be the material basis for the stronger effect of TSW than
TSF. Finally, the molecular docking were performed to visualize the binding modes. Our findings suggested that both TSW and
TSF could effectively ameliorate the progression of
COPD and might be used for the treatment of
COPD.