Abstract | BACKGROUND: MATERIALS AND METHODS: RESULTS: The ALP activity was increased when miR-17-5p mimics were transfected, whereas the miR-17-5p inhibitor reduced ALP activity (p < 0.05). The number and average area of mineral nodes in the miR-17-5p mimic group was larger than those in the corresponding control and NC groups (p < 0.05). The number and average area of the mineral nodes in the miR-17-5p inhibitor group were smaller than those in the corresponding control and NC groups (p < 0.05). Bmp2, OC, Col1a and Runx2 were higher in the miR-17-5p mimics group compared to those in the control and NC groups. ANKH expression was decreased in VSMCs with the miR-17-5p mimics and increased in VSMCs with miR-17-5p inhibitor. ANKH siRNA intervention also promoted mineralization. The miR-17-5p expression was upregulated and ANKH was down-regulated in the aortic arteries with calcification. CONCLUSION:
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Authors | Chao Shi, Jiaorong Tan, Jiancan Lu, Junling Huang, Xiangqi Li, Jiahong Xu, Xing Wang |
Journal | Current neurovascular research
(Curr Neurovasc Res)
Vol. 19
Issue 1
Pg. 108-116
( 2022)
ISSN: 1875-5739 [Electronic] United Arab Emirates |
PMID | 35297350
(Publication Type: Journal Article)
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Copyright | Copyright© Bentham Science Publishers; For any queries, please email at [email protected]. |
Chemical References |
- ANKH protein, human
- Core Binding Factor Alpha 1 Subunit
- MIRN17 microRNA, human
- MicroRNAs
- Phosphate Transport Proteins
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Topics |
- Cell Differentiation
- Cells, Cultured
- Core Binding Factor Alpha 1 Subunit
(genetics, metabolism)
- Humans
- MicroRNAs
(metabolism)
- Myocytes, Smooth Muscle
- Osteogenesis
(genetics)
- Phosphate Transport Proteins
(genetics, metabolism)
- Vascular Calcification
(metabolism)
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