Abstract |
Cell death and inflammation are intimately linked during mastitis due to Staphylococcus aureus (S. aureus). Pyroptosis, a programmed necrosis triggered by gasdermin protein family, often occurs after inflammatory caspase activation. Many pathogens invade host cells and activate cell-intrinsic death mechanisms, including pyroptosis, apoptosis, and necroptosis. We reported that bovine mammary epithelial cells (MAC-T) respond to S. aureus by NOD-like receptor protein 3 (NLRP3) inflammasome activation through K+ efflux, leading to the recruitment of apoptosis-associated speck-like protein (ASC) and the activation of caspase-1. The activated caspase-1 cleaves gasdermin D (GSDMD) and forms a N-terminal pore forming domain that drives swelling and membrane rupture. Membrane rupture results in the release of the pro-inflammatory cytokines IL-18 and IL-1β, which are activated by caspase-1. Can modulate GSDMD activation by NLRP3-dependent caspase-1 activation and then cause pyroptosis of bovine mammary epithelial cells.
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Authors | Xiaozhou Wang, Mingchao Liu, Na Geng, Yongzhen Du, Zhaoming Li, Xin Gao, Bo Han, Jianzhu Liu, Yongxia Liu |
Journal | Veterinary research
(Vet Res)
Vol. 53
Issue 1
Pg. 10
(Feb 05 2022)
ISSN: 1297-9716 [Electronic] England |
PMID | 35123552
(Publication Type: Journal Article)
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Copyright | © 2022. The Author(s). |
Chemical References |
- Inflammasomes
- Interleukin-1beta
- NLR Family, Pyrin Domain-Containing 3 Protein
- NLR Proteins
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Topics |
- Animals
- Cattle
- Epithelial Cells
(metabolism)
- Female
- Inflammasomes
(metabolism)
- Interleukin-1beta
(metabolism)
- NLR Family, Pyrin Domain-Containing 3 Protein
(metabolism)
- NLR Proteins
- Pyroptosis
- Staphylococcus aureus
(metabolism)
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