PA and OA induce abnormal glucose metabolism by inhibiting KLF15 in adipocytes.

Abstract

BACKGROUND:

Obesity-induced elevated serum free fatty acids (FFAs) levels result in the occurrence of type 2 diabetes mellitus (T2DM). However, the molecular mechanism remains largely enigmatic. This study was to explore the effect and mechanism of KLF15 on FFAs-induced abnormal glucose metabolism.

METHODS:

Levels of TG, TC, HDL-C, LDL-C, and glucose were measured by different assay kits. qRT-PCR and Western Blot were used to detect the levels of GPR120, GPR40, phosphorylation of p38 MAPK, KLF15, and downstream factors.

RESULTS:

KLF15 was decreased in visceral adipose tissue of obesity subjects and high-fat diet (HFD) mice. In HFD mice, GPR120 antagonist significantly promoted KLF15 protein expression level and phosphorylation of p38 MAPK, meanwhile reduced the blood glucose levels. While, blocking GPR40 inhibited the KLF15 expression. In 3T3-L1 adipocytes, 1500 μM PA inhibited KLF15 through a GPR120/P-p38 MAPK signal pathway, and 750 μM OA inhibited KLF15 mainly through GPR120 while not dependent on P-p38 MAPK, ultimately resulting in abnormal glucose metabolism. Unfortunately, GPR40 didn't contribute to PA or OA-induced KLF15 reduction.

CONCLUSIONS:

Both PA and OA inhibit KLF15 expression through GPR120, leading to abnormal glucose metabolism in adipocytes. Notably, the inhibition of KLF15 expression by PA depends on phosphorylation of p38 MAPK.

• Authors: Cuizhe Wang, Xiaolong Chu, Yuchun Deng, Jingzhou Wang, Tongtong Qiu, Jiaojiao Zhu, Xin Yang, Chongge Pan, Jianyu Xiong, Jianxin Xie, Yongsheng Chang, Jun Zhang
• Journal: Nutrition & metabolism (Nutr Metab (Lond)) Vol. 18 Issue 1 Pg. 100 (Nov 21 2021) ISSN: 1743-7075 [Print] England
• PMID: 34802421 (Publication Type: Journal Article)
• Copyright: © 2021. The Author(s).