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Comparative analysis of the miRNA-mRNA regulation networks in turbot (Scophthalmus maximus L.) following Vibrio anguillarum infection.

Abstract
MicroRNAs could not only regulate posttranscriptional silencing of target genes in eukaryotic organisms, but also have positive effect on their target genes as well. These microRNAs have been reported to be involved in mucosal immune responses to pathogen infection in teleost. Therefore, we constructed the immune-related miRNA-mRNA networks in turbot intestine following Vibrio anguillarum infection. In our results, 1550 differentially expressed (DE) genes and 167 DE miRNAs were identified. 113 DE miRNAs targeting 89 DE mRNAs related to immune response were used to construct miRNA-mRNA interaction networks. Functional analysis showed that target genes were associated with synthesis and degradation of ketone bodies, mucin type O-Glycan biosynthesis, homologous recombination, biotin metabolism, and intestinal immune network for IgA production that were equivalent to the function of IgT and IgM in fish intestine. Finally, 10 DE miRNAs and 7 DE mRNAs were selected for validating the accuracy of high-throughput sequencing results by qRT-PCR. The results of this study will provide valuable information for the elucidation of the regulation mechanisms of miRNA-mRNA interactions involved in disease resistance in teleost mucosal immune system.
AuthorsChengbin Gao, Xin Cai, Min Cao, Qiang Fu, Ning Yang, Xiaoli Liu, Beibei Wang, Chao Li
JournalDevelopmental and comparative immunology (Dev Comp Immunol) Vol. 124 Pg. 104164 (11 2021) ISSN: 1879-0089 [Electronic] United States
PMID34129850 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2021 Elsevier Ltd. All rights reserved.
Chemical References
  • Fish Proteins
  • MicroRNAs
  • RNA, Messenger
Topics
  • Animals
  • Disease Resistance (genetics, immunology)
  • Fish Diseases (genetics, immunology)
  • Fish Proteins (genetics, immunology)
  • Flatfishes (genetics, immunology)
  • Gene Expression Regulation (immunology)
  • Gene Regulatory Networks (immunology)
  • Immunity, Mucosal (genetics)
  • MicroRNAs (genetics, immunology)
  • RNA, Messenger (genetics, immunology)
  • Vibrio (physiology)
  • Vibrio Infections (genetics, immunology, veterinary)

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