Abstract | BACKGROUND: METHODS: The expression of USP14 in placental tissues from healthy donors and preeclampsia patients were determined by quantitative reverse transcription PCR assay. The protein levels of targeted genes were evaluated by Western blotting assay. Small interfering RNA-mediated gene knockdown was used to reduce USP14 expression in trophoblast cell lines. RESULTS: The expression levels of USP14 and proinflammatory cytokine were substantially upregulated in placental tissues from preeclampsia patients. Knockdown or inhibition of USP14 significantly abrogated hypoxia/reoxygenation-induced upregulation of nuclear factor kappa B (NF-κB) activation and proinflammatory cytokine production. CONCLUSION: Our results suggested that USP14 promotes proinflammatory cytokine production through activation of NF-κB. Developing drugs targeting USP14 may be beneficial for the prevention or treatment of patients with preeclampsia.
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Authors | Yingzi Zhao, Fang Zong |
Journal | Immunity, inflammation and disease
(Immun Inflamm Dis)
Vol. 9
Issue 3
Pg. 1016-1024
(09 2021)
ISSN: 2050-4527 [Electronic] England |
PMID | 34089575
(Publication Type: Journal Article)
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Copyright | © 2021 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd. |
Chemical References |
- NF-kappa B
- USP14 protein, human
- Ubiquitin Thiolesterase
|
Topics |
- Cell Line
- Female
- Humans
- MAP Kinase Signaling System
- NF-kappa B
- Placenta
- Pregnancy
- Signal Transduction
- Trophoblasts
(metabolism)
- Ubiquitin Thiolesterase
|