IL-17 Affects the Progression, Metastasis, and Recurrence of Laryngeal Cancer via the Inhibition of Apoptosis through Activation of the PI3K/AKT/FAS/FASL Pathways.

Abstract

BACKGROUND:

Cytokines play important roles in the development and prognosis of laryngeal cancer (LC). Interleukin-17 (IL-17) from a distinct subset of CD4+ T cells may significantly induce cancer-elicited inflammation to prevent tumor immune surveillance.

METHODS:

The expression levels of IL-17 were examined among 60 patients with LC. Immunofluorescence colocalization experiments were performed to verify the localization of IL-17 and FAS/FASL in Hep-2 and Tu212 cells. The role of IL-17 was determined using siRNA techniques in the LC cell line.

RESULTS:

In the LC patients, cytokines were dysregulated in LC tissues compared with normal tissues. It was found that IL-17 was overexpressed in a cohort of 60 LC tumors paired with nontumor tissues. Moreover, high IL-17 expression was significantly associated with the advanced T category, the late clinical stage, differentiation, lymph node metastasis, and recurrence. In addition, the time course expression of FAS and FASL was observed after stimulation and treatment with the IL-17 stimulator. Finally, in vitro experiments demonstrated that IL-17 functioned as an oncogene by inhibiting the apoptosis of LC cells via the PI3K/AKT/FAS/FASL pathways.

CONCLUSIONS:

In summary, these findings demonstrated for the first time the role of IL-17 as a tumor promoter and a prometastatic factor in LC and indicated that IL-17 may have an oncogenic role and serve as a potential prognostic biomarker and therapeutic target in LC.

• Authors: Yang Song, Ming Yang, Hongjian Zhang, Yan Sun, Ye Tao, Huihui Li, Jing Zhang, Yuncheng Li, Jianming Yang
• Journal: Journal of immunology research (J Immunol Res) Vol. 2020 Pg. 2953191 ( 2020) ISSN: 2314-7156 [Electronic] Egypt
• PMID: 33415169 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 Yang Song et al.

Chemical References

• Cytokines
• Fas Ligand Protein
• Interleukin-17
• fas Receptor
• Proto-Oncogene Proteins c-akt

Topics

• Adult
• Aged
• Aged, 80 and over
• Apoptosis (genetics, immunology)
• Cell Line, Tumor
• Cytokines (metabolism)
• Disease Progression
• Fas Ligand Protein (metabolism)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Immunomodulation (genetics)
• Interleukin-17 (genetics, metabolism)
• Laryngeal Neoplasms (genetics, metabolism, pathology)
• Lymphocyte Activation (genetics, immunology)
• Male
• Middle Aged
• Neoplasm Grading
• Neoplasm Staging
• Phosphatidylinositol 3-Kinases (metabolism)
• Proto-Oncogene Proteins c-akt (metabolism)
• ROC Curve
• Signal Transduction
• T-Lymphocytes (immunology, metabolism)
• Young Adult
• fas Receptor (metabolism)