Abstract | PURPOSE: METHODS: Participants from three randomized, placebo-controlled crossover studies, with increased endogenous or exogenous ketone bodies, were included. Study 1: patients with type 1 diabetes (T1D) (n = 9) were investigated after a) insulin deprivation and lipopolysaccharide (LPS) injection and b) insulin-controlled euglycemia. Study 2: patients with T1D (n = 9) were investigated after a) total insulin deprivation for 9 hours and b) insulin-controlled euglycemia. Study 3: Healthy adults (n = 9) were examined during a) 3-hydroxybutyrate (OHB) infusion and b) saline infusion. Plasma FGF21 was measured with immunoassay in serial samples. RESULTS: Circulating OHB levels were significantly increased to 1.3, 1.5, and 5.5 mmol/l in the three studies, but no correlations with FGF21 levels were found. Also, no correlations between FGF21, insulin, or glucagon were found. Insulin deprivation and LPS injection resulted in increased plasma FGF21 levels at t = 120 min (p = .005) which normalized at t = 240 min. CONCLUSION: We found no correlation between circulating FGF21 levels and levels of ketone bodies. This suggests that it is not ketosis per se which controls FGF21 production, but instead a rather more complex regulatory mechanism. TRIAL REGISTRATION: clinicaltrials.gov ID number: Study 1: NCT02157155 (5/6-2014), study 2: NCT02077348 (4/3-2014), and study 3: NCT02357550 (6/2-2015).
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Authors | Esben Stistrup Lauritzen, Mads Vandsted Svart, Thomas Voss, Niels Møller, Mette Bjerre |
Journal | Endocrine research
(Endocr Res)
Vol. 46
Issue 1
Pg. 20-27
(Feb 2021)
ISSN: 1532-4206 [Electronic] England |
PMID | 33074729
(Publication Type: Journal Article, Randomized Controlled Trial)
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Chemical References |
- FGF21 protein, human
- Insulin
- Ketone Bodies
- Lipopolysaccharides
- Fibroblast Growth Factors
- 3-Hydroxybutyric Acid
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Topics |
- 3-Hydroxybutyric Acid
(administration & dosage, blood)
- Adult
- Cross-Over Studies
- Diabetes Mellitus, Type 1
(blood)
- Female
- Fibroblast Growth Factors
(blood)
- Humans
- Insulin
(metabolism)
- Ketone Bodies
(administration & dosage, blood)
- Lipopolysaccharides
(administration & dosage)
- Male
- Middle Aged
- Young Adult
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