Protective effect of picroside I against hepatic fibrosis in mice via sphingolipid metabolism, bile acid biosynthesis, and PPAR signaling pathway.

Abstract

Picroside I, a hepatoprotectant isolated from Picrorhiza kurroa Royle ex Benth and P. scrophulariiflora Pennell, can reduce liver injury in humans and animals. However, its anti-fibrosis effect remains elusive. This work aimed to explore the mechanism underlying the hepatoprotective effect of picroside I against hepatic fibrosis. Male mice (12 mice per group) were randomly divided into six groups: the control group; the model group, which received thioacetamide (TAA); the positive group, which received TAA + S-(5'-adenosyl)-l-methionine (SAMe, 10 mg/kg); the low-dose group, which received TAA + picroside I (25 mg/kg); the middle-dose group, which received TAA + picroside I (50 mg/kg); and the high-dose group, which received TAA + picroside I (75 mg/kg). Serum biochemical indicators were detected, and histological evaluation was performed. Metabolomics and proteomic analyses were conducted via liquid-chromatography coupled with tandem mass spectrometry (LC-MS/MS). Data showed that picroside I could decrease the serum levels of alanine transaminase (ALT), aspartate transaminase (AST), collagen type IV (CIV), N-terminal peptide of type III procollagen (PIIINP), laminin (LN), and hyaluronic acid (HA) and reduced fibrosis area. Picroside I altered metabolomic profiles, including energy, lipid, and glutathione (GSH) metabolism, in ice with fibrosis. Additionally, 25 differentially expressed proteins in the picroside I high-dose-treated group were reversed relative to in the model group. These proteins were involved in the sphingolipid signaling pathway, primary bile acid biosynthesis, and peroxisome proliferator-activated receptor (PPAR) signaling pathway. Moreover, this study revealed how picroside I could protect against TAA-induced liver fibrosis in mice. Results indicated that picroside I can serve as a candidate drug for hepatic fibrosis.

• Authors: Kai Xiong, Mengge Shi, Tong Zhang, Han Han
• Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (Biomed Pharmacother) Vol. 131 Pg. 110683 (Nov 2020) ISSN: 1950-6007 [Electronic] France
• PMID: 32942155 (Publication Type: Journal Article)
• Copyright: Copyright © 2020 The Author(s). Published by Elsevier Masson SAS.. All rights reserved.

Chemical References

• Bile Acids and Salts
• Cinnamates
• Iridoid Glucosides
• Peroxisome Proliferator-Activated Receptors
• Sphingolipids
• picroside I

Topics

• Animals
• Bile Acids and Salts (biosynthesis)
• Cinnamates (pharmacology, therapeutic use)
• Dose-Response Relationship, Drug
• Iridoid Glucosides (pharmacology, therapeutic use)
• Lipid Metabolism (drug effects, physiology)
• Liver Cirrhosis (metabolism, pathology, prevention & control)
• Male
• Mice
• Mice, Inbred C57BL
• Peroxisome Proliferator-Activated Receptors (metabolism)
• Random Allocation
• Signal Transduction (drug effects, physiology)
• Sphingolipids (metabolism)
• Treatment Outcome