Abstract | BACKGROUND: METHODS: First, the IBS-D rat model was established by chemical stimulation and acute and chronic pressure stimulation. Then, different dose of ginger were administrated to IBS-D rats and evaluate the defecation frequency, fecal water content (FWC) and abdominal withdrawal reflex (AWR) scores in IBS-D rats. Further, the IBS-D rats were sacrificed to collecte the colonic tissues to evaluate the effect of ginger administration on its pathology and changes of pro-inflammatory factors, and changes of NF-κB pathway. Second, the ginger was taken to HPLC analysis and 6-gingerol was choosen to further experiment. Then, IBS-D rats were treated with different dose of 6-gingerol, and the behavioral evaluation were to evaluate the effect of 6-gingerol on IBS-D rats. Further, colonic epithelial cells (CECs) were collectted and to evaluate the effect of 6-gingerol on the expression of inflammatory factors and changes of NF-κB pathway. RESULTS: The IBS-D rat model was successfully established by chemical stimulation and acute and chronic pressure stimulation. And ginger treatment significantly reduced the defecation frequency, fecal water content and AWR scores in IBS-D rats. Histopathological analysis showed that ginger treatment can significantly reduce colonic edema and promote the recovery of inflammation in IBS-D rats, and the effect is equivalent to rifaximin. Elisa and RT-qPCR showed that ginger inhibited the expression of proinflammatory factors (TNF-α, IL-6, iNOS) in IBS-D rats. Western blot showed IkBα was up-regulated while p-p65 was inhibited under ginger treatment. HPLC analysis showed that 6-gingerol was the main component of ginger, which could improve clinical symptoms in IBS-D rats. Western blot and RT-qPCR showed that 6-gingerol inhibited the expression of proinflammatory factors (TNF-α, IL-6, iNOS) in CECs, and inhibition of IκBα degradation and phosphorylation of p65 involved in NF-κB pathway. CONCLUSION:
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Authors | Changrong Zhang, Yongquan Huang, Peiwu Li, Xinlin Chen, Fengbin Liu, Qiuke Hou |
Journal | BMC complementary medicine and therapies
(BMC Complement Med Ther)
Vol. 20
Issue 1
Pg. 279
(Sep 14 2020)
ISSN: 2662-7671 [Electronic] England |
PMID | 32928188
(Publication Type: Journal Article)
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Chemical References |
- Plant Extracts
- Acetic Acid
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Topics |
- Acetic Acid
- Animals
- China
- Diarrhea
(drug therapy)
- Disease Models, Animal
- Zingiber officinale
- Inflammation
(drug therapy)
- Irritable Bowel Syndrome
(drug therapy)
- Male
- Plant Extracts
(pharmacology)
- Rats
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