Dihydroquercetin attenuates lipopolysaccharide-induced acute lung injury through modulating FOXO3-mediated NF-κB signaling via miR-132-3p.

Abstract

BACKGROUND:

Dihydroquercetin (DHQ) is a potent flavonoid which has been demonstrated to have multiple biological activities including anti-inflammation activity, antioxidant activity as well as anti-cancer activity etc. Recently, many studies have focused on the antioxidant activity of DHQ. However, the use of the anti-inflammation activity of DHQ in acute lung injury (ALI) has not been reported.

METHODS:

Cell viability was examined by CCK-8 assay. The relative expression of miR-132-3p, FOXO3 were detected by qPCR. The levels of TNF-α, IL-6 and IL-1β were detected using enzyme-linked immunosorbent assay. The amount of apoptosis cells was detected by flow cytometry. The protein levels of Bcl-2, Bax, p-p65 and p-IκBα were measured by western blot.

RESULTS:

We found that DHQ-induced the expression of miR-132-3p in LPS-induced ALI. Overexpression of miR-132-3p resulted in the inhibition of FOXO3 expression and then suppressed FOXO3-activated NF-κB pathway, attenuating LPS-induced inflammatory response and apoptosis.

CONCLUSION:

We demonstrated FOXO3 to be a target of miR-132-3p, and DHQ could induce the expression of miR-132-3p, relieving LPS-induced ALI via miR-132-3p/FOXO3/NF-κB axis, providing a promising therapeutic target for ALI.

• Authors: Jian-Hua Liu, Liang Cao, Chang-Hong Zhang, Chen Li, Zhi-Hua Zhang, Qi Wu
• Journal: Pulmonary pharmacology & therapeutics (Pulm Pharmacol Ther) Vol. 64 Pg. 101934 (10 2020) ISSN: 1522-9629 [Electronic] England
• PMID: 32805387 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2020 Elsevier Ltd. All rights reserved.

Chemical References

• FOXO3 protein, human
• Forkhead Box Protein O3
• Lipopolysaccharides
• MIRN132 microRNA, human
• MicroRNAs
• NF-kappa B
• Quercetin
• taxifolin

Topics

• Acute Lung Injury (chemically induced, drug therapy)
• Forkhead Box Protein O3 (genetics)
• Humans
• Lipopolysaccharides (toxicity)
• MicroRNAs (genetics)
• NF-kappa B
• Quercetin (analogs & derivatives)