Sepsis causes organ dysfunction due to an
infection, and it may impact the central nervous system.
Neuroinflammation and oxidative stress are related to brain dysfunction after
sepsis. Both processes affect microglia activation,
neurotrophin production, and long-term cognition.
Fish oil (FO) is an anti-inflammatory compound, and
lipoic acid (LA) is a universal
antioxidant substance. They exert neuroprotective roles when administered alone. We aimed at determining the effect of FO+LA combination on microglia activation and brain dysfunction after
sepsis. Microglia cells from neonatal pups were co-treated with
lipopolysaccharide (LPS) and FO or LA, alone or combined, for 24 h.
Cytokine levels were measured. Wistar rats were subjected to
sepsis by cecal
ligation and perforation (CLP) and treated orally with FO, LA, or FO+LA. At 24 h after surgery, the hippocampus, prefrontal cortex, and total cortex were obtained and assayed for levels of
cytokines,
myeloperoxidase (MPO) activity,
protein carbonyls,
superoxide dismutase (SOD), and
catalase (CAT) activity. At 10 days after surgery,
brain-derived neurotrophic factor (
BDNF) levels were determined and behavioral tests were performed. The combination diminished in vitro levels of pro-inflammatory
cytokines. The combination reduced TNF-α in the cortex, IL-1β in the prefrontal cortex, as well as MPO activity, and decreased
protein carbonyls formation in all structures. The combination enhanced
catalase activity in the prefrontal cortex and hippocampus, elevated
BDNF levels in all structures, and prevented behavioral impairment. In summary, the combination was effective in preventing cognitive damage by reducing
neuroinflammation and oxidative stress and increasing
BDNF levels.