Abstract | OBJECTIVE: The aim of the study is to investigate the apoptotic mechanism in salivary glands in the rat experimental periodontitis model. DESIGN: A rat periodontitis model was prepared by using a ligature around the second upper molar. In the salivary (parotid and submandibular) glands and blood samples, putative apoptotic factors and pathway molecules were investigated in vivo and in vitro. RESULTS: Four weeks of ligation ( chronic periodontitis) demonstrated significant apoptotic atrophy of the salivary gland, but one week of ligation (initial periodontitis) did not. In the blood plasma, tumor necrosis factor-α (TNF-α) was increased in the periodontitis model, but interleukin-1β and -6 were not. TNF-α receptor type 1, which has an intracellular apoptotic pathway, was expressed in the salivary glands of rats. Western blot analysis of cultured rat primary salivary gland cells demonstrated that TNF-α induced cleavage of poly (ADP-ribose) polymerase (PARP) and caspase-3 in a dose-dependent manner, indicating apoptosis induction. Additionally, we found increment of circulating lymphocytes in the model. Expression of mRNA and immunoreactive cells for the B lymphocyte marker CD19 were increased in the salivary gland in the model. Western blotting showed that coculture with extracted B cells from the periodontitis model increased cleaved PARP in salivary gland cells. CONCLUSIONS:
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Authors | T Shikayama, J Fujita-Yoshigaki, M Sago-Ito, M Nakamura-Kiyama, M Naniwa, S Hitomi, I Ujihara, S Kataoka, N Yada, W Ariyoshi, M Usui, K Nakashima, K Ono |
Journal | Archives of oral biology
(Arch Oral Biol)
Vol. 117
Pg. 104775
(Sep 2020)
ISSN: 1879-1506 [Electronic] England |
PMID | 32512258
(Publication Type: Journal Article)
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Copyright | Copyright © 2020 Elsevier Ltd. All rights reserved. |
Chemical References |
- Tumor Necrosis Factor-alpha
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Topics |
- Animals
- Apoptosis
- B-Lymphocytes
(cytology)
- Chronic Periodontitis
(pathology)
- Rats
- Salivary Glands
(pathology)
- Tumor Necrosis Factor-alpha
(blood)
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