Recombinant human irisin regulated collagen II, matrix metalloproteinase-13 and the Wnt/β-catenin and NF-κB signaling pathways in interleukin-1β-induced human SW1353 cells.

Abstract

Osteoarthritis (OA) is a degenerative joint disease that seriously affects the quality of life of patients. Irisin has been reported to regulate bone metabolism via the cellular autocrine mechanism and play a protective role in rat OA. In the present study, a SW1353 chondrosarcoma cell line was treated with interleukin (IL)-1β and irisin. The present study evaluated cell viability, expression levels of collagen II (Col II) and matrix metalloproteinase-13 (MMP-13), and activity of the Wnt/β-catenin and NF-κB signaling pathways in treated SW1353 cells. The present results suggested that IL-1β could decrease Col II expression and increase MMP-13 expression at both the mRNA and protein levels, and also activate the Wnt/β-catenin and NF-κB signaling pathways in SW1353 cells. By contrast, irisin was identified to reverse the effects of IL-1β in IL-1β-induced SW1353 cells. The present results suggested that irisin treatment may have a cartilage-protective role in an IL-1β-induced SW1353 cell model.

• Authors: Xiaojun Li, Yibin Liu, Qiang Liu, Sa Wang, Yang Ma, Qunhua Jin
• Journal: Experimental and therapeutic medicine (Exp Ther Med) Vol. 19 Issue 4 Pg. 2879-2886 (Apr 2020) ISSN: 1792-0981 [Print] Greece
• PMID: 32256772 (Publication Type: Journal Article)
• Copyright: Copyright: © Li et al.