Abstract | OBJECTIVE: MATERIALS AND METHODS: A total of 30 clean-grade Sprague-Dawley rats were divided into three groups, including agomiR-146a group (n=10), antagomiR-146a group (n=10) and negative control group (NC, n=10). The model was successfully established via a one-time injection of sodium urate into ankle joint cavity. Subsequently, agomiR-146a (10 μL), antagomiR-146a (10 μL) and normal saline (10 μL) were intrathecally injected into rats in the three groups at 1 h before injection and 12 h, 24 h, 48 h and 72 h after injection, respectively. The ankle joint swelling index, joint dysfunction index and joint inflammation index of rats in the three groups were closely monitored. After 72 h of observation, the rats were euthanized, and synovial tissues were collected from the knee joint. The expression and distribution of nuclear factor-κB (NF-κB) in synovial tissues were detected using the immunohistochemical method. Meanwhile, the expression levels of inflammatory factors, including tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1) and interleukin-6 (IL-6) were detected via enzyme-linked immunosorbent assay. Furthermore, the mRNA and protein expression levels of TLR4 and MyD88 were detected via quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) and Western blotting, respectively. RESULTS: No statistically significant differences in the joint swelling index, joint dysfunction index, joint inflammation index, TLR4 and MyD88 and related inflammatory factors were found between the NC group and antagomiR-146a group. Compared with the NC group, agomiR-146a group showed markedly reduced ankle joint swelling index (p<0.05). Meanwhile, joint landing behavior and inflammatory swelling were significantly relieved in the agomiR-146a group (p<0.05). The mRNA and protein expression levels of TLR4 and MyD88 were remarkably decreased as well (p<0.05). Furthermore, the expression and distribution of NF-κB in synovial tissues of agomiR-146a group was markedly reduced when compared with the NC group (p<0.05). In addition, agomiR-146a group exhibited significantly lower expression levels of inflammatory factors (TNF-α, IL-1 and IL-6) in synovial tissues (p<0.05). CONCLUSIONS: MiR-146a alleviates joint inflammation of acute arthritis in rats through the TLR4/MyD88/NF-κB signaling pathway, which may become a new therapeutic target.
|
Authors | X Chen, Q Gao, L Zhou, Y Wang, R-R Sun, Z-Y Zhang |
Journal | European review for medical and pharmacological sciences
(Eur Rev Med Pharmacol Sci)
Vol. 23
Issue 21
Pg. 9230-9237
(Nov 2019)
ISSN: 2284-0729 [Electronic] Italy |
PMID | 31773674
(Publication Type: Journal Article)
|
Chemical References |
- Inflammation Mediators
- MIRN146a microRNA, rat
- MicroRNAs
- Myeloid Differentiation Factor 88
- NF-kappa B
- Toll-Like Receptor 4
- Uric Acid
|
Topics |
- Animals
- Ankle Joint
(physiopathology)
- Arthritis, Gouty
(chemically induced, metabolism, physiopathology)
- Inflammation
(physiopathology)
- Inflammation Mediators
(metabolism)
- Male
- MicroRNAs
(agonists, antagonists & inhibitors, physiology)
- Myeloid Differentiation Factor 88
(biosynthesis)
- NF-kappa B
(biosynthesis)
- Rats
- Signal Transduction
(physiology)
- Synovial Membrane
(metabolism)
- Time Factors
- Toll-Like Receptor 4
(biosynthesis)
- Uric Acid
|