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Development of a model of ischemic heart disease using cardiomyocytes differentiated from human induced pluripotent stem cells.

Abstract
Ischemic heart disease remains the largest cause of death worldwide. Accordingly, many researchers have sought curative options, often using laboratory animal models such as rodents. However, the physiology of the human heart differs significantly from that of the rodent heart. In this study, we developed a model of ischemic heart disease using cardiomyocytes differentiated from human induced pluripotent stem cells (hiPS-CMs). After optimizing the conditions of ischemia, including the concentration of oxygen and duration of application, we evaluated the consequent damage to hiPS-CMs. Notably, exposure to 2% oxygen, 0 mg/ml glucose, and 0% fetal bovine serum increased the percentage of nuclei stained with propidium iodide, an indicator of membrane damage, and decreased cellular viability. These conditions also decreased the contractility of hiPS-CMs. Furthermore, ischemic conditioning increased the mRNA expression of IL-8, consistent with observed conditions in the in vivo heart. Taken together, these findings suggest that our hiPS-CM-based model can provide a useful platform for human ischemic heart disease research.
AuthorsHeng Wei, Chen Wang, Rui Guo, Ken Takahashi, Keiji Naruse
JournalBiochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 520 Issue 3 Pg. 600-605 (12 10 2019) ISSN: 1090-2104 [Electronic] United States
PMID31623826 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.
Chemical References
  • CXCL8 protein, human
  • Interleukin-8
  • RNA, Messenger
Topics
  • Animals
  • Cell Differentiation
  • Cell Line
  • Cell Survival
  • Cells, Cultured
  • Gene Expression
  • Humans
  • Induced Pluripotent Stem Cells (pathology)
  • Interleukin-8 (genetics)
  • Models, Cardiovascular
  • Myocardial Contraction
  • Myocardial Ischemia (etiology, pathology, physiopathology)
  • Myocytes, Cardiac (pathology)
  • RNA, Messenger (genetics, metabolism)
  • Rats

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