Label-Free Nanoplasmonic Biosensing of Cancer Biomarkers for Clinical Diagnosis.

Abstract

Biosensing of cancer biomarkers enabling early diagnosis of cancer constitutes an essential tool for clinical intervention and application of novel therapies against cancer disease. Optical biosensor instruments as point-of-care (POC) devices and operating under label-free scheme have demonstrated to provide fast, simple, and high-sensitivity assays even at home care environment. Nanoplasmonic biosensors are thought to be a powerful tool for detection of complex analytes of relevant clinical applications. Using high-throughput fabrication techniques, large surface patterned with gold nanodisk structures is obtained showing surface sensitivities with limit of detection (LOD) in the order of picomolar concentration range. Here, we describe two major assay methodologies used for detection of lung and colorectal cancer, respectively. Particularly, we have selected a complementary hybridization DNA/RNA assay for the assessment of two miRNAs (miRNA-210 and miRNA-205) for detection of lung cancer. However, for colorectal cancer we present the detection of four tumor-associated antigen (TAA) biomarkers (MAPKAPK3, PIM-1, STK4, and GTF2B) as possible TAA targets for autoantibody production. Strategies for detecting these biomarkers in real samples such as serum are also presented, demonstrating the capabilities of these assays to be transferred to real clinical settings.

• Authors: Alejandro Portela, Enelia C Peláez, Olalla Calvo-Lozano, Mari C Estévez, Laura M Lechuga
• Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol) Vol. 2027 Pg. 115-140 ( 2019) ISSN: 1940-6029 [Electronic] United States
• PMID: 31309477 (Publication Type: Journal Article)

Chemical References

• Antigens, Neoplasm
• Biomarkers, Tumor
• Gold

Topics

• Antigens, Neoplasm (blood, immunology)
• Biomarkers, Tumor (blood, immunology)
• Biosensing Techniques (instrumentation, methods)
• Colorectal Neoplasms (blood, diagnosis, immunology)
• Gold (chemistry)
• High-Throughput Screening Assays (instrumentation, methods)
• Humans
• Limit of Detection
• Lung Neoplasms (blood, diagnosis, immunology)
• Metal Nanoparticles (chemistry)
• Point-of-Care Testing