Abstract |
A new electrochemical immunosensor for cancer cell detection based on a specific interaction between the metastasis-related antigen of epithelial cell adhesion molecule ( EpCAM) on the cell membrane and its monoclonal antibody (Anti- EpCAM) immobilized on a gold electrode has been developed. The amino-terminated polyamidoamine dendrimer (G6 PAMAM) was first covalently attached to the 3-mercaptopropionic acid (MPA)-functionalized gold electrode to obtain a thin film, and then completely carboxylated by succinic anhydride (SA). Next, the Anti- EpCAM was covalently bound with the G6 PAMAM to obtain a stable recognition layer. In the presence of the EpCAM expressing hepatocellular carcinomas cell line of HepG2, the specific immune recognition (Anti- EpCAM/ EpCAM) led to an obvious change of the electron transfer ability. The properties of the layer-by-layer assembly process was examined by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The final determination of HepG2 cells was performed in the presence of the reversible [Fe(CN)6]3-/4- redox couple using impedance technique. Based on the advantages of PAMAM nanomaterial and immune reaction, a linear response to HepG2 cells ranging from 1 × 104 to 1 × 106 cells mL-1 with a calculated detection limit of 2.1 × 103 cells mL-1 was obtained. We expect this method can provide a potential tool for cancer cell monitoring and protein expression analysis.
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Authors | Jianguo Xu, Xinxin Wang, Chao Yan, Wei Chen |
Journal | Sensors (Basel, Switzerland)
(Sensors (Basel))
Vol. 19
Issue 8
(Apr 19 2019)
ISSN: 1424-8220 [Electronic] Switzerland |
PMID | 31010258
(Publication Type: Journal Article)
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Chemical References |
- Antibodies, Monoclonal
- Dendrimers
- EPCAM protein, human
- Epithelial Cell Adhesion Molecule
- PAMAM Starburst
- Poly(amidoamine)
- Polyamines
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Topics |
- Antibodies, Monoclonal
(chemistry, immunology)
- Biosensing Techniques
- Cell Membrane
(chemistry, immunology)
- Dendrimers
(chemistry)
- Dielectric Spectroscopy
- Epithelial Cell Adhesion Molecule
(chemistry, genetics, immunology)
- Hep G2 Cells
- Humans
- Immunoassay
- Liver Neoplasms
(diagnosis, genetics, immunology)
- Nanostructures
(chemistry)
- Polyamines
(chemistry)
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