Abstract | OBJECTIVE: METHODS: A total of 64 male SD rats were randomized into normal control, model, medication and scalp-acupuncture groups (n=16 rats in each group). The focal CI model was established by middle cerebral artery occlusion. Intraperitoneal injection of Ammonium Pyrrolidine Dithiocarbamate (100 mg•kg-1•d-1) was administrated for rats in the medication group, once a day for 7 days. For rats of the scalp-acupuncture group, the acupuncture needles were rapidly inserted into bilateral Dingnieqianxiexian (MS6), followed by twirling the needles at 100 cycles/min for 1 min, once again every 10 min during 20 min' needle retention. The treatment was conducted once a day for 7 days. The neurologic deficit score (0-4 points, impaired consciousness, death, etc.) and neurological function score (motor, sensory and sensory tests, 0-10 points) were assessed according to Longa's (1989) and Schabitz's (2004) methods, respectively. The expression levels of IL-10 mRNA and IL-6 mRNA were detected by fluorescence quantitative PCR, and the expression of TNF-α was detected by immunohistochemistry. RESULTS: After modeling, the neurologic deficit and neurological function scores and the expression levels of IL-10 mRNA, IL-6 mRNA and TNF-α protein in the parahippocampus were significantly increased in the model group than in the normal control group (P<0.01). Following the intervention, the neurologic deficit and neurological function scores as well as IL-6 mRNA and TNF-α protein expression were significantly down-regulated in both scalp-acupuncture and medication groups (P<0.05), and the expression of IL-10 mRNA was obviously increased (P<0.05) relevant to the model group. CONCLUSION: Scalp-acupuncture can improve neurologic function in CI rats, which is related to its effects in up-regulating the expression of IL-10, then down-regulating the expression of IL-6 and TNF-α (reducing inflammatory response) in the parahippocampal gyrus.
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Authors | Jin-Hai Wang, Xiao-Zheng Du, Ting-Zhuo Zhang, Xing-Lan Li, Min Zhao, Ying-Cun Bao, Qi Yan, Zhen-Chang Zhang, Hua Jiang, Liang Tian, Bo Yuan |
Journal | Zhen ci yan jiu = Acupuncture research
(Zhen Ci Yan Jiu)
Vol. 44
Issue 3
Pg. 183-8
(Mar 25 2019)
ISSN: 1000-0607 [Print] China |
PMID | 30945500
(Publication Type: Journal Article)
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Chemical References |
- Il6 protein, rat
- Interleukin-6
- RNA, Messenger
- Tumor Necrosis Factor-alpha
- Interleukin-10
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Topics |
- Acupuncture Therapy
- Animals
- Interleukin-10
- Interleukin-6
- Male
- Parahippocampal Gyrus
- RNA, Messenger
- Rats
- Rats, Sprague-Dawley
- Scalp
- Tumor Necrosis Factor-alpha
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