MicroRNA 10a induces glioma tumorigenesis by targeting myotubularin-related protein 3 and regulating the Wnt/β-catenin signaling pathway.

Abstract

MicroRNAs are involved in the regulation of tumor formation. A previous study suggested that miR-10a promotes glioma cell migration and invasion. However, the effect of miR-10a on the proliferation of glioma cells remains unknown. In this study, we demonstrated that miR-10a promoted proliferation and reduced apoptosis in glioma cells by directly targeting the 3'-UTR of myotubularin-related protein 3 (MTMR3). miR-10a enhanced, while MTMR3 weakened, the growth of glioma in vivo. Ectopic expression of MTMR3 neutralized the effect of miR-10a on glioma. Furthermore, miR-10a and MTMR3 regulated β-catenin expression and genes downstream of the Wnt/β-catenin signaling pathway, such as Bcl-2, c-myc, p-c-Jun, and cleaved caspase-3, to affect the proliferation ability and apoptosis of glioma cells. In conclusion, our results indicated that miR-10a regulated cell proliferation and apoptosis by directly targeting MTMR3 and could function as a prognostic factor for progression of glioma.

• Authors: Yan Yan, Hua Yan, Qin Wang, Le Zhang, Ying Liu, Haimiao Yu
• Journal: The FEBS journal (FEBS J) Vol. 286 Issue 13 Pg. 2577-2592 (07 2019) ISSN: 1742-4658 [Electronic] England
• PMID: 30927504 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2019 Federation of European Biochemical Societies.

Chemical References

• MIRN10 microRNA, human
• MicroRNAs
• beta Catenin
• MTMR3 protein, human
• Protein Tyrosine Phosphatases, Non-Receptor

Topics

• Adult
• Aged
• Apoptosis
• Brain Neoplasms (genetics, metabolism, pathology)
• Carcinogenesis (genetics, metabolism)
• Cell Line, Tumor
• Cell Proliferation
• Female
• Gene Expression Regulation, Neoplastic
• Glioma (genetics, metabolism, pathology)
• Humans
• Male
• MicroRNAs (genetics, metabolism)
• Middle Aged
• Protein Tyrosine Phosphatases, Non-Receptor (genetics, metabolism)
• Wnt Signaling Pathway
• beta Catenin (genetics, metabolism)