Prostate cancer (PCa) is one of the most common
malignancies worldwide, and with a limited number of treatments for this type of
cancer, its incidence is rapidly increasing. Patients presenting with PCa are likely to experience disease recurrence, which represents a considerable clinical challenge.
MicroRNAs (
miRNAs) have been widely characterized as a critical regulator in a number of types of
cancer, including PCa. miRNA-744 (miR-744) has been reported to be involved in
cancer regulation; however, its role in PCa remained poorly understood. In a recent study, it was demonstrated that miR-744 was overexpressed in prostate tissue from PCa patients when compared with the surrounding tissues, and knockdown of miR-744 resulted in reduced cell growth. In addition, an increased population of apoptotic cells was detected upon miR-744 knockdown, together with a decrease in cell proliferation. Cell cycle analysis demonstrated a higher number of cells in the G1 phase and lower numbers in the S phase following miR-744 silencing. The levels of key
proteins involved in cell cycle progression (
cyclin D1,
cyclin-dependent kinase 4, and
proliferating cell nuclear antigen) were increased, whereas those
proteins responsible for cell cycle inhibition (
cyclin-dependent kinase inhibitor p21) were decreased. The
tumor suppressor liver
kinase B1 (LKB1) was revealed to be a potential target of miR-744, suggesting its potential mechanism of action. LKB1 levels were negatively correlated with miR-744, and LKB1 was indicated to be a direct target of miR-744. Furthermore, it was revealed that by targeting LKB1, miR-744 may regulate
adenosine monophosphate-activated
protein kinase (AMPK); the AMPK signaling pathway was activated by miR-744 knockdown, with subsequent inhibition of the
mammalian target of rapamycin (mTOR) signaling pathway. Taken together, these results demonstrated that miR-744 promoted cell growth through the AMPK signaling pathway, by targeting LKB1. The present study revealed a novel insight into the biological function of miR-744 in PCa, and that miR-744 may be a potential therapeutic target.