Abstract |
The effects of long-term oral administration of magnesium sulfate and insulin on hyperglycemia were investigated using Akt2 and IRS1 gene expression methods in streptozotocin-induced diabetic rats. Fifty rats were randomly divided into five experimental groups: 1, non-diabetic control (NDC); 2, Mg2+-treated non-diabetic control (Mg-NDC); 3, chronic diabetic (CD); 4, Mg2+-treated chronic diabetic (Mg-CD); and 5, insulin-treated chronic diabetic (Ins-CD). Streptozotocin was used to induce diabetes. The Mg-CD and Mg-NDC groups received 10 g/l of MgSO4 added to drinking water. The Ins-CD group received 2.5 U/kg of insulin twice a day. Blood glucose level and body weight were measured every week. The intraperitoneal glucose tolerance test (IPGTT) was performed after 16 weeks. MgSO4 administration improved the blood glucose level and IPGTT. It also increased Akt2 and IRS1 genes as well as protein expression. Insulin lowered the blood glucose level and increased IRS1 gene and protein expression, but did not affect Akt2 gene and protein expression. Glucose reduction after Mg therapy may be mediated, at least partially, via IRS1 and Akt2 genes and protein stimulation. In insulin-treated rats, insulin resistance was not significant due to the absence of Akt2 gene expression.
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Authors | Mitra Kamran, Fatemeh Kharazmi, Kianoosh Malekzadeh, Ardeshir Talebi, Fatemah Khosravi, Nepton Soltani |
Journal | Biological trace element research
(Biol Trace Elem Res)
Vol. 190
Issue 2
Pg. 396-404
(Aug 2019)
ISSN: 1559-0720 [Electronic] United States |
PMID | 30519800
(Publication Type: Journal Article)
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Chemical References |
- Blood Glucose
- Hypoglycemic Agents
- Insulin
- Insulin Receptor Substrate Proteins
- Irs1 protein, rat
- Streptozocin
- Magnesium Sulfate
- Akt2 protein, rat
- Proto-Oncogene Proteins c-akt
- Glucose
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Topics |
- Administration, Oral
- Animals
- Blood Glucose
(drug effects)
- Dose-Response Relationship, Drug
- Glucose
(administration & dosage)
- Glucose Tolerance Test
- Hyperglycemia
(chemically induced, drug therapy, metabolism)
- Hypoglycemic Agents
(administration & dosage, pharmacology)
- Injections, Intraperitoneal
- Insulin
(administration & dosage, pharmacology)
- Insulin Receptor Substrate Proteins
(genetics, metabolism)
- Magnesium Sulfate
(administration & dosage, pharmacology)
- Male
- Proto-Oncogene Proteins c-akt
(genetics, metabolism)
- Rats
- Rats, Wistar
- Real-Time Polymerase Chain Reaction
- Streptozocin
- Structure-Activity Relationship
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