Lipoteichoic Acid Isolated from Lactobacillus plantarum Maintains Inflammatory Homeostasis through Regulation of Th1- and Th2-Induced Cytokines.

Abstract

Lipoteichoic acid isolated from Lactobacillus plantarum K8 (pLTA) alleviates lipopolysaccharide (LPS)-induced excessive inflammation through inhibition of TNF-α and interleukin (IL)-6. In addition, pLTA increases the survival rate of mice in a septic shock model. In the current study, we have found that pLTA contributes to homeostasis through regulation of pro- and anti-inflammatory cytokine production. In detail, pLTA decreased the production of IL-10 by phorbol-12-myristate-13-acetate (PMA)-differentiated THP-1 cells stimulated with prostaglandin E2 (PGE-2) and LPS. However, TNF-α production which was inhibited by PGE-2+LPS increased by pLTA treatment. The regulatory effects of IL-10 and TNF-α induced by PGE-2 and LPS in PMA-differentiated THP-1 cells were mediated by pLTA, but not by other LTAs isolated from either Staphylococcus aureus (aLTA) or L. sakei (sLTA). Further studies revealed that pLTA-mediated IL-10 inhibition and TNF-α induction in PGE-2+LPS-stimulated PMA-differentiated THP-1 cells were mediated by dephosphorylation of p38 and phosphorylation of c-Jun N-terminal kinase (JNK), respectively. Reduction of pLTA-mediated IL-10 inhibited the metastasis of breast cancer cells (MDA-MB-231), which was induced by IL-10 or conditioned media prepared from PGE-2+LPS-stimulated PMA-differentiated THP-1 cells. Taken together, our data suggest that pLTA contributes to inflammatory homeostasis through induction of repressed pro-inflammatory cytokines as well as inhibition of excessive anti-inflammatory cytokines.

• Authors: Ji Eun Ahn, Hangeun Kim, Dae Kyun Chung
• Journal: Journal of microbiology and biotechnology (J Microbiol Biotechnol) Vol. 29 Issue 1 Pg. 151-159 (Jan 28 2019) ISSN: 1738-8872 [Electronic] Korea (South)
• PMID: 30415531 (Publication Type: Journal Article)

Chemical References

• IL10 protein, human
• Lipopolysaccharides
• Teichoic Acids
• Tumor Necrosis Factor-alpha
• Interleukin-10
• lipoteichoic acid
• JNK Mitogen-Activated Protein Kinases
• p38 Mitogen-Activated Protein Kinases

Topics

• Cell Line, Tumor
• Cell Survival (drug effects)
• Humans
• Inflammation (chemically induced, metabolism, prevention & control)
• Interleukin-10 (antagonists & inhibitors, metabolism)
• JNK Mitogen-Activated Protein Kinases (metabolism)
• Lactobacillus plantarum (chemistry)
• Lipopolysaccharides (pharmacology)
• Phosphorylation (drug effects)
• Species Specificity
• Teichoic Acids (pharmacology)
• Th1-Th2 Balance (drug effects)
• Tumor Necrosis Factor-alpha (metabolism)
• p38 Mitogen-Activated Protein Kinases (metabolism)