Antiobesity Effect of Prebiotic Polyphenol-Rich Grape Seed Flour Supplemented with Probiotic Kefir-Derived Lactic Acid Bacteria.

Abstract

The interaction between prebiotics and probiotics may exert synergistic health benefits. This study investigated the combined effects of polyphenol-rich wine grape seed flour (GSF), a prebiotic, and lactic acid bacteria (LAB) derived from kefir, a probiotic, on obesity-related metabolic disease in high-fat diet (HFD) induced obese (DIO) mice. DIO mice were fed with HFD with 6% microcrystalline cellulose (CON) or HFD supplemented with GSF (5% or 10% GSF), HFD with LAB orally administrated (LAB), or HFD with a combination of GSF and LAB orally administrated (GSF+LAB) for 9 weeks. The vehicle, saline, was also orally administered to the CON and GSF groups. In comparison to CON, all GSF and LAB groups showed a reduction ( P < 0.05) in HF-induced weight gain, liver and adipose tissue weights, plasma lipid concentrations, insulin resistance, and glucose intolerance. The combination of 10% GSF and LAB showed synergistic effects ( P < 0.05) on body weight gain, plasma insulin and total cholesterol concentrations, and cecum propionate contents. Plasma zonulin and cecum propionate concentrations and intestinal FXR gene expression were ( P < 0.05) correlated with body weight gain. A pathway analysis of microarray data of adipose tissue showed that the combination of GSF and LAB affected genes involved in metabolic and immunological diseases, including inflammasome complex assembly ( P < 0.05). In conclusion, a combination of GSF and LAB inhibited HF-induced obesity and inflammation via alterations in intestinal permeability and adipocyte gene expression.

• Authors: Yun-Ju Cho, Hyeon Gyu Lee, Kun-Ho Seo, Wallace Yokoyama, Hyunsook Kim
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 66 Issue 47 Pg. 12498-12511 (Nov 28 2018) ISSN: 1520-5118 [Electronic] United States
• PMID: 30392364 (Publication Type: Journal Article)

Chemical References

• Anti-Obesity Agents
• Insulin
• Plant Extracts
• Polyphenols
• Prebiotics
• Cholesterol

Topics

• Animals
• Anti-Obesity Agents (administration & dosage, analysis)
• Cholesterol (blood)
• Diet, High-Fat (adverse effects)
• Dietary Supplements (analysis)
• Flour (analysis)
• Humans
• Insulin (blood)
• Kefir (microbiology)
• Lactobacillales (genetics, isolation & purification, physiology)
• Male
• Mice
• Mice, Inbred C57BL
• Obesity (drug therapy, metabolism)
• Plant Extracts (administration & dosage, analysis)
• Polyphenols (administration & dosage, analysis)
• Prebiotics (administration & dosage, analysis)
• Probiotics (administration & dosage, chemistry)
• Seeds (chemistry)
• Synbiotics (administration & dosage, analysis)
• Vitis (chemistry)