[Effects of FBI-1 silencing on proliferation and apoptosis of triple-negative breast cancer cell line MDA-MB-231].

Abstract

This work aimed to observe the effects of short hairpin RNA (shRNA)-silenced FBI-1 (factor that binds to the inducer of short transcripts of human immunodeficiency virus-1) on proliferation and apoptosis of triple-negative breast cancer cell line MDA-MB-231. qRT-PCR and Western blot analysis were applied to detect the mRNA and/or protein expression of FBI-1, Bcl-2, Bax, cleaved-Caspase 3 and Survivin. RNA interference method was used to silence FBI-1 expression in MDA-MB-231 cells. CCK-8 and colony formation assay were employed to detect the cell proliferation. Flow cytometry was employed for examining cell apoptosis. In vivo tumorigenicity of MDA-MB-231 cells was detected by tumor transplantation in nude mice. The results showed that the mRNA and protein expressions of FBI-1 were higher in MDA-MB-231 cells compared with those in normal human mammary epithelial cells MCF-10A. FBI-1 gene silencing inhibited proliferation and induced apoptosis of MDA-MB-231 cells in vitro, together with decreased Bcl-2 and Survivin protein expression, increased Bax protein expression and activated Caspase 3. Moreover, FBI-1 gene silencing inhibited the tumorigenesis of MDA-MB-231 cells in vivo. These results suggest that silencing of FBI-1 gene inhibits proliferation, induces apoptosis and suppresses the tumorigenesis of MDA-MB-231 cells.

• Authors: Mao-Jian Chen, Li Wang, Wei-Ping Yang, Qing-Hong Qin, Qi-Xing Tan, Bin Lian, Chang-Yuan Wei
• Journal: Sheng li xue bao : [Acta physiologica Sinica] (Sheng Li Xue Bao) Vol. 70 Issue 5 Pg. 497-503 (Oct 25 2018) ISSN: 0371-0874 [Print] China
• PMID: 30377688 (Publication Type: Journal Article)

Chemical References

• BAX protein, human
• BCL2 protein, human
• BIRC5 protein, human
• DNA-Binding Proteins
• Proto-Oncogene Proteins c-bcl-2
• RNA, Messenger
• RNA, Small Interfering
• Survivin
• Transcription Factors
• ZBTB7A protein, human
• bcl-2-Associated X Protein
• CASP3 protein, human
• Caspase 3

Topics

• Animals
• Apoptosis
• Caspase 3 (metabolism)
• Cell Line, Tumor
• Cell Proliferation
• DNA-Binding Proteins (genetics)
• Humans
• Mice
• Mice, Nude
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• RNA Interference
• RNA, Messenger
• RNA, Small Interfering
• Survivin (metabolism)
• Transcription Factors (genetics)
• Triple Negative Breast Neoplasms (genetics, pathology)
• bcl-2-Associated X Protein (metabolism)